|Tested species reactivity||Human, Yeast|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Tissue / cell preparation (Mitotic human HeLa cell cytosolic lysate).|
|Storage buffer||0.1M tris glycine, pH 7.4, with 0.15M NaCl, 40% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunocytochemistry (ICC)||5-15 µg/ml|
|Immunofluorescence (IF)||5-15 ug/ml|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91855 has been successfully used in immunocytochemistry, Western blot and ELISA applications.
The MA1-91855 immunogen is: Tissue / cell preparation (Mitotic human HeLa cell cytosolic lysate).
Progression of cells from interphase to mitosis involves alteration in cell structures and activities. The transition of G2 to M phase is induced by M phase-promoting factor, or MPF. In M phase, many proteins are phosphorylated directly by MPF or indirectly by kinases activated by MPF. These M-phase phosphoproteins (MMPs or MPHOSPHs) permit disassembly of interphase structures and generation of M-phase enzymatic activities and structures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.