Western blot analysis of MRE11 using A) 30 µg Jurkat whole cell extract (B) 30 µg Raji whole cell extract and C) 30 µg NCI-H929 whole cell extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a MRE11 monoclonal antibody (Product # MA1-23253) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Amino acids 182-582 of Mre11 expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Functional Assay (FN)||Assay-dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1:1000-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23253 detects MRE11 in Human samples.
MA1-23253 has been successfully used in ELISA, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry procedures.
MA1-23253 immunogen corresponds to Amino acids 182-582 of Mre11 expressed in E. coli..
Mre11 is an 80 kDa protein that acts as a Mn+2 dependent double stranded DNA 3' to 5' exonuclease and a single stranded DNA endonuclease. It is part of a larger complex with Rad50, p95 (Nibrin) and possibly other associated proteins involved in the nucleolytic end-processing of double strand breaks and telomere maintenance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.