Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human tonsil stained using a MRF2 polyclonal antibody (Product # PA5-23380) at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Human, Non-human primate, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (513 DPEKDNETDQGSNSEK 528) of MRF2 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MRF2 belongs to the ARID (A-T rich interaction domain) family of transcription factors. The ARID (A-T Rich Interaction Domain) is a helix-turn-helix motif-based DNA-binding domain, which is conserved in all eukaryotes and diagnostic of a family that includes fifteen distinct human proteins. It shows important roles in development, tissue-specific gene expression and proliferation control. This protein has a nonredundant function during growth and in the development of the reproductive system. MRF2 is essential for accumulation of lipid stores in postnatal life. Northern blot analysis shows expression in mouse lung, heart, small intestine, kidney, muscle, and brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.