Western blot analysis of MRG15 / MORF4L1 in Mouse Liver (A), Rat Liver (B) and Pig Liver (C) lysate (35µg protein in RIPA buffer). Samples were probed with the MRG15 / MORF4L1 antibody (Product # PA5-18195, 0.3µg / ml ) for 1 hour. Western blot was detected by chemiluminescence.
|Tested species reactivity||Human, Mouse, Rabbit|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (APKQDPKPKFQEG) corresponding to the N-terminus amino acids of MORF4L1|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4ug/ml|
|Western Blot (WB)||0.1-0.3ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, human, porcine and rat based on sequence homology.
Cellular senescence is the terminal non-dividing state that normal cells enter following completion of their proliferative potential. Fusions of immortal human cell lines with each other have led to their assignment to one of four complementation groups. Mortality factor 4 (MORF4) was identified as the lead member of a family of transcription factor-like proteins that reverses this immortal phenotype. Like other members in this family, MORF4 is localized to the nucleus and possesses transcription factor-like motifs such as helix-loop-helix and a leucine zipper motif that might allow it to form transcriptionally active homo- or heterodimers. MORF4 has been shown to bind to the transcription corepressors mSin3A and TLE, suggesting that together, these complexes may play a role in transcriptional repression of genes that lead to cellular senescence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.