|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||recombinant Human MRP8|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-90712 detects MRP8 from human samples.
MA1-90712 has been successfully used in flow cytometry, Western blot and ELISA applications. Western blot detects a band ~82 kDa. A suggested positive control for this product is neutrophil lysate (particularly neutrophils from spleen).
The MA1-90712 immunogen is: recombinant Human MRP8
MRP-8 and MRP-14 are cytosolic calcium-binding proteins of 8kDa and 14kDa that form a heterodimer. MRP-8 and MRP-14 are expressed in peripheral blood monocytes, neutrophils and keratinocytes (mature). Expression is lost on tissue maturation of monocytes to macrophages. MRP-14 may be associated with monocyte and neutrophil activation and the accumulation of these cells in inflammatory sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.