OriGene

MRPS7 Antibody (UMAB156), UltraMAB™ Mouse Monoclonal

Product Image Gallery

MRPS7 Antibody in Western Blot (WB) — Western blot analysis of HCT116 cell lysate (35 µg) by using anti-MRPS7 monoclonal antibody.

MRPS7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human composit of placenta using anti-MRPS7 clone UMAB156 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM800053 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110°C. The image shows membranous and cytoplasmic staining in trophoblast cells.

MRPS7 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of MCF-7 cells using anti-MRPS7 mouse monoclonal antibody (UM800053, green, 1:50). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2000

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:200

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

UMAB156

Immunogen

Human recombinant protein fragment corresponding to amino acids 38-242 of human MRPS7 produced in E.coli.

Conjugate

Unconjugated

Form

liquid

Concentration

0.5-1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. In the prokaryotic ribosome, the comparable protein is thought to play an essential role in organizing the 3' domain of the 16 S rRNA in the vicinity of the P- and A-sites. Pseudogenes corresponding to this gene are found on chromosomes 8p and 12p.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 28S ribosomal protein S7, mitochondrial; 30S ribosomal protein S7 homolog; bMRP-27a; bMRP27a; Mitochondrial small ribosomal subunit protein uS7m; MRP-S7

Gene Aliases: bMRP27a; MRP-S; MRP-S7; MRPS7; RP-S7; RPMS7; S7mt

UniProt ID: (Human) Q9Y2R9

Entrez Gene ID: (Human) 51081

Molecular Function: RNA binding protein nucleic acid binding ribosomal protein