Immunofluorescence analysis of MSH2 was performed using 90% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MSH2 (FE11) Mouse Monoclonal Antibody (33-7900) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||C-terminal fragment of the human MSH2 protein|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 21 publications below|
|Miscellaneous PubMed (MISC)||See 6 publications below|
|Immunohistochemistry (IHC)||See 4 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
MSH2 is involved in DNA repair as a mismatch repair protein, and mutations of MSH2 are found in approximately 50% of inherited non polyposis colorectal carcinoma (HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the western world.
IP-MS enrichment of MSH2 (LFQ intensity): MSH2 was enriched 105-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and MSH2 antibody (Part No. 33-7900). See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dynamic modulation of phosphoprotein expression in ovarian cancer xenograft models.
33-7900 was used in immunohistochemistry - paraffin section to utilize ovarian cancer xenograft models to study dynamic modulation of phosphoprotein expression
|Koussounadis A,Langdon SP,Um I,Kay C,Francis KE,Harrison DJ,Smith VA||BMC cancer (16:null)||2016|
Prognostic Implication of M2 Macrophages Are Determined by the Proportional Balance of Tumor Associated Macrophages and Tumor Infiltrating Lymphocytes in Microsatellite-Unstable Gastric Carcinoma.
33-7900 was used in immunohistochemistry - paraffin section to assess how proportional balance of tumor associated macrophages and tumor infiltrating lymphocytes in microsatellite-unstable gastric carcinoma is a prognostic indicator of M2 macrophages
|Kim KJ,Wen XY,Yang HK,Kim WH,Kang GH||PloS one (10:null)||2015|
Association between IHC and MSI testing to identify mismatch repair-deficient patients with ovarian cancer.
33-7900 was used in immunohistochemistry - paraffin section to determine the association of microsatellite instability with mismatch repair proteins in epithelial cancer samples
|Lee JH,Cragun D,Thompson Z,Coppola D,Nicosia SV,Akbari M,Zhang S,McLaughlin J,Narod S,Schildkraut J,Sellers TA,Pal T||Genetic testing and molecular biomarkers (18:229)||2014|
Filiform polyposis: A benign entity? Case report and literature review.
33-7900 was used in immunohistochemistry - paraffin section in the first case of filiform polyposis associated with adenomas developed on filiform polyps and invasive colonic adenocarcinoma in a patient without history of inflammatory bowel disease.
|Boulagnon C,Jazeron JF,Diaz-Cives A,Ehrhard F,Bouché O,Diebold MD||Pathology, research and practice (210:189)||2014|
|Not Applicable||Not Cited||
Clinical and histomolecular endometrial tumor characterization of patients at-risk for Lynch syndrome in South of Brazil.
33-7900 was used in immunohistochemistry - paraffin section to measure the frequency of mismatch repair deficiencies in women diagnosed with endometrial cancer who are at-risk for Lynch syndrome
|Cossio SL,Koehler-Santos P,Pessini SA,Mónego H,Edelweiss MI,Meurer L,Errami A,Coffa J,Bock H,Saraiva-Pereira ML,Ashton-Prolla P,Prolla JC||Familial cancer (9:131)||2010|
Clinical implications of microsatellite instability and MLH1 gene inactivation in sporadic insulinomas.
33-7900 was used in immunohistochemistry - paraffin section to discuss biomarkers for insulinomas
|Mei M,Deng D,Liu TH,Sang XT,Lu X,Xiang HD,Zhou J,Wu H,Yang Y,Chen J,Lu CM,Chen YJ||The Journal of clinical endocrinology and metabolism (94:3448)||2009|
Expression of hMLH1 and hMSH2 proteins in pleomorphic adenoma of minor salivary glands: relationship with clinical and histologic findings.
33-7900 was used in immunohistochemistry - paraffin section to assess the relationship between clinicopathological features and expression of hMLH1 and hMSH2 proteins in pleomorphic adenoma of minor salivary glands
|Tobón-Arroyave SI,Flórez-Moreno GA,Jaramillo-Cárdenas JF,Arango-Uribe JD,Isaza-Guzmán DM,Rendón-Henao J||Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics (108:227)||2009|
Impact of microsatellite instability (MSI) on survival in high grade endometrial carcinoma.
33-7900 was used in immunohistochemistry - paraffin section to correlate microstellite instability and other clinicopathologic markers with survival of patients with high grade endometrial carcinoma
|Arabi H,Guan H,Kumar S,Cote M,Bandyopadhyay S,Bryant C,Shah J,Abdul-Karim FW,Munkarah AR,Ali-Fehmi R||Gynecologic oncology (113:153)||2009|
Sampling strategies for tissue microarrays to evaluate biomarkers in ovarian cancer.
33-7900 was used in immunohistochemistry - paraffin section to discuss the use of microarrays to study ovarian cancer
|Permuth-Wey J,Boulware D,Valkov N,Livingston S,Nicosia S,Lee JH,Sutphen R,Schildkraut J,Narod S,Parker A,Coppola D,Sellers T,Pal T||Cancer epidemiology, biomarkers and prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology (18:28)||2009|
|Not Applicable||Not Cited||
Oncoprotein Bcl-2 and microsatellite instability are associated with disease-free survival and treatment response in colorectal cancer.
33-7900 was used in immunohistochemistry - paraffin section to determine the expression of Bcl-2, hMLH1, and hMSH2 in patients with advanced colorectal cancer and correlate their expression with therapeutic response and disease outcome
|Bendardaf R,Lamlum H,Ristamäki R,Syrjänen K,Pyrhönen S||Oncology reports (20:999)||2008|
Thymidylate synthase and microsatellite instability in colorectal cancer: implications for disease free survival, treatment response and survival with metastases.
33-7900 was used in immunohistochemistry - paraffin section to evaluate thymidylate synthase and microsatellite instability in colorectal cancer
|Bendardaf R,Lamlum H,Ristamäki R,Korkeila E,Syrjänen K,Pyrhönen S||Acta oncologica (Stockholm, Sweden) (47:1046)||2008|
|Not Applicable||210 mg/l||
Hereditary nonpolyposis colorectal cancer in endometrial cancer patients.
33-7900 was used in immunohistochemistry - paraffin section to identify the number of hereditary nonpolyposis colorectal cancer patients that exist among endometrial cancer patients
|Yoon SN,Ku JL,Shin YK,Kim KH,Choi JS,Jang EJ,Park HC,Kim DW,Kim MA,Kim WH,Lee TS,Kim JW,Park NH,Song YS,Kang SB,Lee HP,Jeong SY,Park JG||International journal of cancer (122:1077)||2008|
Overexpression of hMSH2 and hMLH1 protein in certain gastric cancers and their surrounding mucosae.
33-7900 was used in immunohistochemistry - paraffin section to quantify expression levels of mismatch repair proteins hMSH2, hMLH1, PCNA and Ki67 in the mucosae collected from the patients with gastric cancer
|Li M,Liu L,Wang Z,Wang L,Liu Z,Xu G,Lu S||Oncology reports (19:401)||2008|
Prognostic value of microsatellite instability determined by immunohistochemical staining of hMSH2 and hMSH6 in urothelial carcinoma of the bladder.
33-7900 was used in immunohistochemistry - paraffin section to detect expression of MMR gene products hMSH2 and hMSH6 in urothelial carcinoma of the bladder
|Mylona E,Zarogiannos A,Nomikos A,Giannopoulou I,Nikolaou I,Zervas A,Nakopoulou L||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (116:59)||2008|
DNA mismatch repair and TP53 defects are early events in uterine carcinosarcoma tumorigenesis.
33-7900 was used in immunohistochemistry - paraffin section to provide evidence that carcinosarcomas are clonal malignancies
|Taylor NP,Zighelboim I,Huettner PC,Powell MA,Gibb RK,Rader JS,Mutch DG,Edmonston TB,Goodfellow PJ||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (19:1333)||2006|
|Not Applicable||Not Cited||
X chromosomal and autosomal loss of heterozygosity and microsatellite instability in human cervical carcinoma.
33-7900 was used in immunohistochemistry - paraffin section to assess patients with pure squamous cell carcinoma of the uterine cervix for loss of heterozygosity and microsatellite instability
|Edelmann J,Richter K,Hänel C,Hering S,Horn LC||International journal of gynecological cancer : official journal of the International Gynecological Cancer Society (16:1248)||2006|
|Not Applicable||Not Cited||
Differential expression of alpha-methylacyl coenzyme A racemase in adenocarcinomas of the small and large intestines.
33-7900 was used in immunohistochemistry - paraffin section to compare alpha-methylacyl coenzyme A racemase expression in primary small intestinal adenocarcinomas and colorectal adenocarcinomas
|Chen ZM,Ritter JH,Wang HL||The American journal of surgical pathology (29:890)||2005|
|Not Applicable||Not Cited||
High thymidylate synthase expression in colorectal cancer with microsatellite instability: implications for chemotherapeutic strategies.
33-7900 was used in immunohistochemistry - paraffin section to study the role of high thymidylate synthase expression in colorectal cancer with microsatellite instability
|Ricciardiello L,Ceccarelli C,Angiolini G,Pariali M,Chieco P,Paterini P,Biasco G,Martinelli GN,Roda E,Bazzoli F||Clinical cancer research : an official journal of the American Association for Cancer Research (11:4234)||2005|
Colonic crypt changes during adenoma development in familial adenomatous polyposis: immunohistochemical evidence for expansion of the crypt base cell population.
33-7900 was used in immunohistochemistry - paraffin section to elucidate how crypt stem cells contribute to the development of colon cancer
|Boman BM,Walters R,Fields JZ,Kovatich AJ,Zhang T,Isenberg GA,Goldstein SD,Palazzo JP||The American journal of pathology (165:1489)||2004|
Microsatellite status and cell cycle associated markers in rectal cancer patients undergoing a combined regimen of 5-FU and CPT-11 chemotherapy and radiotherapy.
33-7900 was used in immunohistochemistry - paraffin section to identify the molecular profiles of patients with rectal cancers treated with neoadjuvant chemotherapy, radiotherapy, and surgery that correlate therapeutic responses
|Charara M,Edmonston TB,Burkholder S,Walters R,Anne P,Mitchell E,Fry R,Boman B,Rose D,Fishel R,Curran W,Palazzo J||Anticancer research (24:3161)||2004|
Increased risk for hereditary nonpolyposis colorectal cancer-associated synchronous and metachronous malignancies in patients with microsatellite instability-positive endometrial carcinoma lacking MLH1 promoter methylation.
33-7900 was used in immunohistochemistry - paraffin section to assess the number and types of malignancies in patients with endometrial carcinoma with and without microsatellite instability
|Buttin BM,Powell MA,Mutch DG,Rader JS,Herzog TJ,Gibb RK,Huettner P,Edmonston TB,Goodfellow PJ||Clinical cancer research : an official journal of the American Association for Cancer Research (10:481)||2004|
Characteristics of hereditary nonpolyposis colorectal cancer patients with double primary cancers in endometrium and colorectum.
33-7900 was used in immunohistochemistry (paraffin) to elucidate the clinicopathologic characteristics of hereditary nonpolyposis colorectal cancer patients who had both endometrial and colorectal cancers.
|Shin SH,Yu EJ,Lee YK,Song YS,Seong MW,Park SS||Obstetrics and gynecology science (58:112)||2015|
A renal metanephric adenoma showing both a 2p16e24 deletion and BRAF V600E mutation: a synergistic role for a tumor suppressor gene on chromosome 2p and BRAF activation?
33-7900 was used in immunohistochemistry to identify and characterize BRAF mutation from patients with metanephric adenomas.
|Dadone B,Ambrosetti D,Carpentier X,Duranton-Tanneur V,Burel-Vandenbos F,Amiel J,Pedeutour F||Cancer genetics (206:347)||2014|
Mutation mismatch repair gene deletions in diffuse large B-cell lymphoma.
33-7900 was used in immunohistochemistry (paraffin) to elucidate the pathogenesis of diffuse large B-cell lymphoma.
|Couronné L,Ruminy P,Waultier-Rascalou A,Rainville V,Cornic M,Picquenot JM,Figeac M,Bastard C,Tilly H,Jardin F||Leukemia and lymphoma (54:1079)||2013|
Immunoexpression of p53 and hMSH2 in oral squamous cell carcinoma and oral dysplastic lesions in Yemen: relationship to oral risk habits and prognostic factors.
33-7900 was used in immunohistochemistry (paraffin) to determine the roles of p53 and hMSH2 proteins in oral squamous cell carcinoma and oral dysplastic lesions.
|Helal Tel A,Fadel MT,El-Thobbani AK,El-Sarhi AM||Oral oncology (48:120)||2012|
Renal medullary carcinomas: histopathologic phenotype associated with diverse genotypes.
33-7900 was used in immunohistochemistry (paraffin) to examine patients with renal medullary carcinoma with and without sickle cell anemia.
|Gatalica Z,Lilleberg SL,Monzon FA,Koul MS,Bridge JA,Knezetic J,Legendre B,Sharma P,McCue PA||Human pathology (42:1979)||2011|
DNA polymerases as potential therapeutic targets for cancers deficient in the DNA mismatch repair proteins MSH2 or MLH1.
33-7900 was used in immunohistochemistry - paraffin section to elucidate how synthetic sickness/lethality affects cancer cells
|Martin SA,McCabe N,Mullarkey M,Cummins R,Burgess DJ,Nakabeppu Y,Oka S,Kay E,Lord CJ,Ashworth A||Cancer cell (17:235)||2010|
Expression of the mismatch repair gene hMLH1 is enhanced in non-small cell lung cancer with EGFR mutations.
33-7900 was used in immunohistochemistry to study the elevated hMLH expression observed in EGFR-mutated non-small cell lung cancer.
|Li M,Zhang Q,Liu L,Lu W,Wei H,Li RW,Lu S||PloS one (8:null)||2013|
|Human||Not Cited||Uncertainty in the utility of immunohistochemistry in mismatch repair protein expression in epithelial ovarian cancer.||Coppola D,Nicosia SV,Doty A,Sellers TA,Lee JH,Fulp J,Thompson Z,Galeb S,McLaughlin J,Narod SA,Schildkraut J,Pal T||Anticancer research (32:4963)||2012|
Frequency of familial colon cancer and hereditary nonpolyposis colorectal cancer (Lynch syndrome) in a large population database.
33-7900 was used in immunohistochemistry to discuss the numbers of colorectal cancer cases based on Amsterdam I and II criteria
|Kerber RA,Neklason DW,Samowitz WS,Burt RW||Familial cancer (4:239)||2005|
Mutations of the BRAF gene in ulcerative colitis-related colorectal carcinoma.
33-7900 was used in immunohistochemistry to determine the function of BRAF in ulcerative colitis-related colorectal carcinogenesis
|Aust DE,Haase M,Dobryden L,Markwarth A,Löhrs U,Wittekind C,Baretton GB,Tannapfel A||International journal of cancer (115:673)||2005|
|Human||Not Cited||Reduced FHIT expression is associated with mismatch repair deficient and high CpG island methylator phenotype colorectal cancer.||Al-Temaimi RA,Jacob S,Al-Ali W,Thomas DA,Al-Mulla F||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (61:627)||2013|
Human gut flora-fermented nondigestible fraction from cooked bean ( Phaseolus vulgaris L.) modifies protein expression associated with apoptosis, cell cycle arrest, and proliferation in human adenocarcinoma colon cancer cells.
33-7900 was used in western blot to assess the effects of fermentation products produced by the human gut flora on human adenocarcinoma colon cancer cells.
|Campos-Vega R,García-Gasca T,Guevara-Gonzalez R,Ramos-Gomez M,Oomah BD,Loarca-Piña G||Journal of agricultural and food chemistry (60:12443)||2012|
|Human||Not Cited||Humans accumulate microsatellite instability with acquired loss of MLH1 protein in hematopoietic stem and progenitor cells as a function of age.||Kenyon J,Fu P,Lingas K,Thomas E,Saurastri A,Santos Guasch G,Wald D,Gerson SL||Blood (120:3229)||2012|