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Immunofluorescence analysis of MSH2 was performed using 90% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MSH2 (FE11) Mouse Monoclonal Antibody (33-7900) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||C-terminal fragment of the human MSH2 protein|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
MSH2 is involved in DNA repair as a mismatch repair protein, and mutations of MSH2 are found in approximately 50% of inherited non polyposis colorectal carcinoma (HNPCC) (Lynch syndrome) cases. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the western world.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Association between IHC and MSI testing to identify mismatch repair-deficient patients with ovarian cancer.
33-7900 was used in immunohistochemistry - paraffin section to determine the association of microsatellite instability with mismatch repair proteins in epithelial cancer samples
|Lee JH,Cragun D,Thompson Z,Coppola D,Nicosia SV,Akbari M,Zhang S,McLaughlin J,Narod S,Schildkraut J,Sellers TA,Pal T||Genetic testing and molecular biomarkers (18:229)||2014|
Filiform polyposis: A benign entity? Case report and literature review.
33-7900 was used in immunohistochemistry - paraffin section in the first case of filiform polyposis associated with adenomas developed on filiform polyps and invasive colonic adenocarcinoma in a patient without history of inflammatory bowel disease.
|Boulagnon C,Jazeron JF,Diaz-Cives A,Ehrhard F,Bouché O,Diebold MD||Pathology, research and practice (210:189)||2014|
Expression of the mismatch repair gene hMLH1 is enhanced in non-small cell lung cancer with EGFR mutations.
33-7900 was used in immunohistochemistry to study the elevated hMLH expression observed in EGFR-mutated non-small cell lung cancer.
|Li M,Zhang Q,Liu L,Lu W,Wei H,Li RW,Lu S||PloS one (8:null)||2013|
|Human||Not Cited||Uncertainty in the utility of immunohistochemistry in mismatch repair protein expression in epithelial ovarian cancer.||Coppola D,Nicosia SV,Doty A,Sellers TA,Lee JH,Fulp J,Thompson Z,Galeb S,McLaughlin J,Narod SA,Schildkraut J,Pal T||Anticancer research (32:4963)||2012|
|Human||Not Cited||Reduced FHIT expression is associated with mismatch repair deficient and high CpG island methylator phenotype colorectal cancer.||Al-Temaimi RA,Jacob S,Al-Ali W,Thomas DA,Al-Mulla F||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (61:627)||2013|
Human gut flora-fermented nondigestible fraction from cooked bean ( Phaseolus vulgaris L.) modifies protein expression associated with apoptosis, cell cycle arrest, and proliferation in human adenocarcinoma colon cancer cells.
33-7900 was used in western blot to assess the effects of fermentation products produced by the human gut flora on human adenocarcinoma colon cancer cells.
|Campos-Vega R,García-Gasca T,Guevara-Gonzalez R,Ramos-Gomez M,Oomah BD,Loarca-Piña G||Journal of agricultural and food chemistry (60:12443)||2012|
|Human||Not Cited||Humans accumulate microsatellite instability with acquired loss of MLH1 protein in hematopoietic stem and progenitor cells as a function of age.||Kenyon J,Fu P,Lingas K,Thomas E,Saurastri A,Santos Guasch G,Wald D,Gerson SL||Blood (120:3229)||2012|
COCA1; DNA mismatch repair protein Msh2; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2; mutS homolog 2, colon cancer, nonpolyposis type 1; mutS protein homolog 2
AI788990; COCA1; FCC1; HNPCC; HNPCC1; LCFS2; MSH2