|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide human MSH6|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Blocking Assay (BLOCK)||See 3 publications below|
MA1-34550 detects MSH6 from human samples.
MA1-34550 has been successfully used in immunohistochemistry (paraffin tissue), Western blot applications. Formalin-fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0, prior to staining. Positive controls: colon carcinoma, tonsil.
The MA1-34550 immunogen is: Synthetic peptide human MSH6
The DNA mismatch repair MSH2/MSH6 heterodimer is responsible for the detection of DNA damage, and may promote apoptosis in normal cells, thus protecting mammals from ultraviolet-induced malignant transformation and hence the prevention of skin cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A surrogate method for assessment of beta(2)-integrin-dependent adhesion of human eosinophils to ICAM-1.
MA1-34550 was used in blocking/activating experiment to investigate the reliability of a novel assay for the detection of eosinophil adhesion
|Zhu X,Subbaraman R,Sano H,Jacobs B,Sano A,Boetticher E,Mu?oz NM,Leff AR||Journal of immunological methods (240:157)||2000|
Cytosolic phospholipase A2 activation is essential for beta 1 and beta 2 integrin-dependent adhesion of human eosinophils.
MA1-34550 was used in blocking/activating experiment to investigate the role of cytosolic phospholipase A2 in eosinophil adhesion
|Zhu X,Mu?oz NM,Kim KP,Sano H,Cho W,Leff AR||Journal of immunology (Baltimore, Md. : 1950) (163:3423)||1999|
Promotion of neutrophil chemotaxis through differential regulation of beta 1 and beta 2 integrins.
MA1-34550 was used in blocking/activating experiment to investigate the effect of beta-glucan for the regulation of neutrophil migration
|Harler MB,Wakshull E,Filardo EJ,Albina JE,Reichner JS||Journal of immunology (Baltimore, Md. : 1950) (162:6792)||1999|