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Immunofluorescence analysis of OXPHOS COMPLEX IV SU (MTCO2) was performed using 90% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MTCO2 (12C4F12) Mouse Monoclonal Antibody (A-6404) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Rat, Non-human primate, Human, Mouse, Chicken|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Full length native Human Cytochrome C oxidase subunit II|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||1µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Cytochrome c oxidase is the fourth complex in the respiratory chain and is responsible for catalyzing the conversion of O2 to H2O. Subunit 2 of the cytochrome c oxidase complex combines with two other subunits (1 and 3) to form a core protein structure that performs many functions of the enzyme. The metallic copper center of this subunit transfers electrons to the heme center of subunit 1, which results in the movement of electrons from cytochrome c to the heme A3 and copper B metallic center of complex IV. Defects in subunit 2 of cytochrome c oxidase can result in COX deficiency, which causes a wide range of symptoms from local myopathy to multiple system pathologies that begin between infancy and adulthood. Abnormalities in this subunit are also associated with tumor development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification of a mitochondrial defect gene signature reveals NUPR1 as a key regulator of liver cancer progression.
A-6404 was used in western blot to investigate the role of NUPR1 during liver carcinogenesis
|Lee YK,Jee BA,Kwon SM,Yoon YS,Xu WG,Wang HJ,Wang XW,Thorgeirsson SS,Lee JS,Woo HG,Yoon G||Hepatology (Baltimore, Md.) (62:1174)||2015|
Mitochondrial Respiratory Dysfunction Induces Claudin-1 Expression via Reactive Oxygen Species-mediated Heat Shock Factor 1 Activation, Leading to Hepatoma Cell Invasiveness.
A-6404 was used in western blot to show that mitochondrial respiratory defects contribute to hepatoma invasiveness.
|Lee JH,Lee YK,Lim JJ,Byun HO,Park I,Kim GH,Xu WG,Wang HJ,Yoon G||The Journal of biological chemistry (290:21421)||2015|
miR-181c regulates the mitochondrial genome, bioenergetics, and propensity for heart failure in vivo.
A-6404 was used in western blot to study the effect of miR-181c administration in rats
|Das S,Bedja D,Campbell N,Dunkerly B,Chenna V,Maitra A,Steenbergen C||PloS one (9:null)||2014|
Glycogen synthase kinase-3 (GSK3) controls deoxyglucose-induced mitochondrial biogenesis in human neuroblastoma SH-SY5Y cells.
A-6404 was used in western blot to study the effect of SB216763 on mitochondrial biogenesis
|Ngamsiri P,Watcharasit P,Satayavivad J||Mitochondrion (14:54)||2014|
Ridaifen-SB8, a novel tamoxifen derivative, induces apoptosis via reactive oxygen species-dependent signaling pathway.
A-6404 was used in western blot to elucidate the mechanism by which RID-SB8 exerts anticancer activity.
|Guo WZ,Shiina I,Wang Y,Umeda E,Watanabe C,Uetake S,Ohashi Y,Yamori T,Dan S||Biochemical pharmacology (86:1272)||2013|
Identification of elongation factor G as the conserved cellular target of argyrin B.
A-6404 was used in western blot to identify elongation factor G as the cellular target of argyrin B.
|Nyfeler B,Hoepfner D,Palestrant D,Kirby CA,Whitehead L,Yu R,Deng G,Caughlan RE,Woods AL,Jones AK,Barnes SW,Walker JR,Gaulis S,Hauy E,Brachmann SM,Krastel P,Studer C,Riedl R,Estoppey D,Aust T,Movva NR,Wang Z,Salcius M,Michaud GA,McAllister G,Murphy LO,Tallarico JA,Wilson CJ,Dean CR||PloS one (7:null)||2012|
Pilot application of iTRAQ to the retinal disease Macular Telangiectasia.
A-6404 was used in western blot to study the application of iTRAQ to the proteomic analysis of the retinal disease Macular Telangiectasia.
|Len AC,Powner MB,Zhu L,Hageman GS,Song X,Fruttiger M,Gillies MC||Journal of proteome research (11:537)||2012|
Mitochondrial proteins, learning and memory: biochemical specialization of a memory system.
A-6404 was used in western blot to study cytochrome c oxidase subunits during learning.
|Solomonia RO,Kunelauri N,Mikautadze E,Apkhazava D,McCabe BJ,Horn G||Neuroscience (194:112)||2011|
Mutations in MTFMT underlie a human disorder of formylation causing impaired mitochondrial translation.
A-6404 was used in western blot to test the requirement of formylation for initiation in human mitochondria.
|Tucker EJ,Hershman SG,Köhrer C,Belcher-Timme CA,Patel J,Goldberger OA,Christodoulou J,Silberstein JM,McKenzie M,Ryan MT,Compton AG,Jaffe JD,Carr SA,Calvo SE,RajBhandary UL,Thorburn DR,Mootha VK||Cell metabolism (14:428)||2011|
Effect of one month duration ketogenic and non-ketogenic high fat diets on mouse brain bioenergetic infrastructure.
A-6404 was used in immunohistochemistry - frozen section to study the effect of ketosis-inducing and non-inducing high fat diets on genes and proteins relevant to brain bioenergetic infrastructures
|Selfridge JE,Wilkins HM,E L,Carl SM,Koppel S,Funk E,Fields T,Lu J,Tang EP,Slawson C,Wang W,Zhu H,Swerdlow RH||Journal of bioenergetics and biomembranes (47:1)||2015|
Cyclin D1 inhibits mitochondrial activity in B cells.
A-6404 was used in immunocytochemistry to study the role of Cyclin D1 in mature B cells.
|Tchakarska G,Roussel M,Troussard X,Sola B||Cancer research (71:1690)||2011|
Perioculomotor cell groups in monkey and man defined by their histochemical and functional properties: reappraisal of the Edinger-Westphal nucleus.
A-6404 was used in immunohistochemistry to investigate the Edinger-Westphal nucleus in primates
|Horn AK,Eberhorn A,Härtig W,Ardeleanu P,Messoudi A,Büttner-Ennever JA||The Journal of comparative neurology (507:1317)||2008|
COII; COXII; Cytochrome c oxidase 2; Cytochrome c oxidase II; MT-CO2; MTCO2
COII; COXII; MT-CO2; MTCO2