Invitrogen

MTHFD2 Monoclonal Antibody (1985CT220.118.31)

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MTHFD2 Antibody in Western Blot (WB) — Western Blot analysis of MTHFD2 using the MTHFD2 Monoclonal Antibody (Product # MA5-37767) at a 1:4000 dilution. Lane 1: Hela whole cell lysate, Lane 2: 293 whole cell lysate, Lane 3: MOLT-4 whole cell lysate, Lane 4: Mouse heart lysate. Lysates/proteins were loaded at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size : 38 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Product Details

MA5-37767

Applications	Tested Dilution	Publications
Western Blot (WB)	1:4,000	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	1985CT220.118.31
Immunogen	KLH conjugated synthetic peptide between 266-299 amino acids from the C-terminal region of human MTHFD2 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target Information

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; Methenyltetrahydrofolate cyclohydrolase; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase; NAD-dependent methylenetetrahydrofolate dehydrogenase

Gene Aliases: AW558851; MTHFD2; NMDMC

UniProt ID: (Human) P13995, (Mouse) P18155

Entrez Gene ID: (Human) 10797, (Mouse) 17768

Function(s)

one-carbon metabolic process folic acid-containing compound biosynthetic process tetrahydrofolate metabolic process folic acid metabolic process oxidation-reduction process metabolic process

Process(es)

magnesium ion binding formate-tetrahydrofolate ligase activity methenyltetrahydrofolate cyclohydrolase activity methylenetetrahydrofolate dehydrogenase (NAD+) activity methylenetetrahydrofolate dehydrogenase (NADP+) activity phosphate ion binding catalytic activity oxidoreductase activity hydrolase activity