Immunofluorescence analysis of MUC4 / Mucin 4 was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MUC4 / Mucin 4 (1G8) Mouse Monoclonal Antibody (354900) at 2ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Bovine, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Nearly all mucosal surfaces are protected by mucin transmembrane glycoproteins, from bronchioles to corneas to reproductive tracts. Mucin 4 (MUC4) is membrane-bound and is important in protecting and lubricating. MUC4 expression is erroneous in many carcinomas and sarcomas, including fibromyoxoid sarcoma and pancreatic adenocarcinoma. Immunohistochemical staining for MUC4 has been discussed as a potential biomarker for detection of these cancers. MUC4 seems to be most predominantly affected in Asian diseases – Taiwanese infertility and endometriosis, as well as gallstone formation in Chinese men, are connected to MUC4, where similar diseases in other populations do not share these MUC4 mutations. Because MUC4 is so important in mucosal surface protection, it is not surprising that it has been implicated as failing in many metastatic cancer invasions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Primary sclerosing epithelioid fibrosarcoma of kidney with variant histomorphologic features: report of 2 cases and review of the literature.
35-4900 was used in immunohistochemistry to present two cases of primary sclerosing epithelioid fibrosarcoma of the kidney
|Ertoy Baydar D,Kosemehmetoglu K,Aydin O,Bridge JA,Buyukeren B,Aki FT||Diagnostic pathology (10:null)||2015|
Tau and PTEN status as predictive markers for response to trastuzumab and paclitaxel in patients with HER2-positive breast cancer.
35-4900 was used in immunohistochemistry - paraffin section to identify biomarkers using HER2-positive advanced breast cancer samples from patients treated with trastuzumab and paclitaxel combination chemotherapy
|Koo DH,Lee HJ,Ahn JH,Yoon DH,Kim SB,Gong G,Son BH,Ahn SH,Jung KH||Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (36:5865)||2015|
Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs.
35-4900 was used in immunohistochemistry - paraffin section to study transcription of lncRNA genes that also encode for miRNAs
|Dhir A,Dhir S,Proudfoot NJ,Jopling CL||Nature structural and molecular biology (22:319)||2015|
|Not Applicable||Not Cited||
Universal antibody conjugation to nanoparticles using the Fc¿ receptor I (Fc¿RI): quantitative profiling of membrane biomarkers.
35-4900 was used in immunocytochemistry to develop a method for universal conjugation of antibodies to lipid-coated nanoparticles using radially oriented FcgammaRIs
|Kim C,Galloway JF,Lee KH,Searson PC||Bioconjugate chemistry (25:1893)||2014|
Prospective randomized controlled study comparing cell block method and conventional smear method for bile cytology.
35-4900 was used in immunohistochemistry to compare the diagnostic efficacies of cell block method and conventional smear method for bile cytology
|Noda Y,Fujita N,Kobayashi G,Ito K,Horaguchi J,Hashimoto S,Koshita S,Ishii S,Kanno Y,Ogawa T,Masu K,Tsuchiya T,Oikawa M,Honda H,Sawai T,Uzuki M,Fujishima F||Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society (25:444)||2013|
Characterization of a novel rat cholangiocarcinoma cell culture model-CGCCA.
35-4900 was used in immunohistochemistry to establish and characterize a thioacetamide-induced culture model of CCA rat cells.
|Yeh CN,Lin KJ,Chen TW,Wu RC,Tsao LC,Chen YT,Weng WH,Chen MF||World journal of gastroenterology (17:2924)||2011|
Prognostic value of MUC4 for mass-forming intrahepatic cholangiocarcinoma after hepatectomy.
35-4900 was used in immunohistochemistry to assess the prognostic value of MUC4 for predicting the overall survival rate for mass-forming-cholangiocarcinoma patients undergoing hepatectomy
|Yeh CN,Pang ST,Wu RC,Chen TW,Jan YY,Chen MF||Oncology reports (21:49)||2009|
AMPK induces MUC5B expression via p38 MAPK in NCI-H292 airway epithelial cells.
35-4900 was used in ELISA to study the mechanism by which AMPK induces MUC5B expression in airway epithelial cells.
|Bae CH,Kim JW,Ye SB,Song SY,Kim YW,Park SY,Kim YD||Biochemical and biophysical research communications (409:669)||2011|
Leptin up-regulates MUC5B expression in human airway epithelial cells via mitogen-activated protein kinase pathway.
35-4900 was used in ELISA to investigate the mechanism of MUC5B upregulation induced by leptin
|Woo HJ,Yoo WJ,Bae CH,Song SY,Kim YW,Park SY,Kim YD||Experimental lung research (36:262)||2010|
|Glycoproteomics of milk: differences in sugar epitopes on human and bovine milk fat globule membranes.||Wilson NL,Robinson LJ,Donnet A,Bovetto L,Packer NH,Karlsson NG||Journal of proteome research (7:3687)||2008|
|Human||Not Cited||Differential regulation of membrane-associated mucins in the human ocular surface epithelium.||Hori Y,Spurr-Michaud S,Russo CL,Argüeso P,Gipson IK||Investigative ophthalmology and visual science (45:114)||2004|