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Immunofluorescence analysis of MUC4 / Mucin 4 was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MUC4 / Mucin 4 (1G8) Mouse Monoclonal Antibody (354900) at 2ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Nearly all mucosal surfaces are protected by mucin transmembrane glycoproteins, from bronchioles to corneas to reproductive tracts. Mucin 4 (MUC4) is membrane-bound and is important in protecting and lubricating. MUC4 expression is erroneous in many carcinomas and sarcomas, including fibromyoxoid sarcoma and pancreatic adenocarcinoma. Immunohistochemical staining for MUC4 has been discussed as a potential biomarker for detection of these cancers. MUC4 seems to be most predominantly affected in Asian diseases – Taiwanese infertility and endometriosis, as well as gallstone formation in Chinese men, are connected to MUC4, where similar diseases in other populations do not share these MUC4 mutations. Because MUC4 is so important in mucosal surface protection, it is not surprising that it has been implicated as failing in many metastatic cancer invasions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Prospective randomized controlled study comparing cell block method and conventional smear method for bile cytology.
35-4900 was used in immunohistochemistry to compare the diagnostic efficacies of cell block method and conventional smear method for bile cytology
|Noda Y,Fujita N,Kobayashi G,Ito K,Horaguchi J,Hashimoto S,Koshita S,Ishii S,Kanno Y,Ogawa T,Masu K,Tsuchiya T,Oikawa M,Honda H,Sawai T,Uzuki M,Fujishima F||Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society (25:444)||2013|
Characterization of a novel rat cholangiocarcinoma cell culture model-CGCCA.
35-4900 was used in immunohistochemistry to establish and characterize a thioacetamide-induced culture model of CCA rat cells.
|Yeh CN,Lin KJ,Chen TW,Wu RC,Tsao LC,Chen YT,Weng WH,Chen MF||World journal of gastroenterology (17:2924)||2011|
AMPK induces MUC5B expression via p38 MAPK in NCI-H292 airway epithelial cells.
35-4900 was used in ELISA to study the mechanism by which AMPK induces MUC5B expression in airway epithelial cells.
|Bae CH,Kim JW,Ye SB,Song SY,Kim YW,Park SY,Kim YD||Biochemical and biophysical research communications (409:669)||2011|
Leptin up-regulates MUC5B expression in human airway epithelial cells via mitogen-activated protein kinase pathway.
35-4900 was used in ELISA to investigate the mechanism of MUC5B upregulation induced by leptin
|Woo HJ,Yoo WJ,Bae CH,Song SY,Kim YW,Park SY,Kim YD||Experimental lung research (36:262)||2010|
|Human||Not Cited||Differential regulation of membrane-associated mucins in the human ocular surface epithelium.||Hori Y,Spurr-Michaud S,Russo CL,Argüeso P,Gipson IK||Investigative ophthalmology & visual science (45:114)||2004|
ascites sialoglycoprotein; ASGP; HSA276359; MUC-4; mucin 4; mucin 4, ASGP; mucin 4, tracheobronchial; mucin-4; pancreatic adenocarcinoma mucin; pre-sialomucin complex; sialomucin ascites sialoglycoprotein-1; sialomucin complex; testis mucin; tracheobronchial; tracheobronchial mucin
4933405I11Rik; ASGP; ASGP-1; HSA276359; MUC-4; MUC4; Psmc