|Tested species reactivity||Cat, Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant mouse MyoD.|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:500|
|Immunoprecipitation (IP)||1 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The epitope of this antibody was mapped to a region within AA 180-189 of mouse MyoD.
Suggested positive control: Rh-30.
The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Synthetic niche to modulate regenerative potential of MSCs and enhance skeletal muscle regeneration.
MA1-41017 was used in western blot to research modulation of regenerative potential of MSCs and enhancement of skeletal muscle regeneration by a synthetic niche
|Pumberger M,Qazi TH,Ehrentraut MC,Textor M,Kueper J,Stoltenburg-Didinger G,Winkler T,von Roth P,Reinke S,Borselli C,Perka C,Mooney DJ,Duda GN,Geißler S||Biomaterials (99:95)||2016|
The role of TORC1 in muscle development in Drosophila.
MA1-41017 was used in western blot to determine the role of mTORC1 in myogenesis using drosophila
|Hatfield I,Harvey I,Yates ER,Redd JR,Reiter LT,Bridges D||Scientific reports (5:null)||2015|
ADP-ribosylation of integrin alpha7 modulates the binding of integrin alpha7beta1 to laminin.
MA1-41017 was used in western blot to study the effect of ADP-ribosylation on the interaction between integrin alpha7beta1 and laminin
|Zhao Z,Gruszczynska-Biegala J,Zolkiewska A||The Biochemical journal (385:309)||2005|
Roles of peroxisome proliferator-activated receptors delta and gamma in myoblast transdifferentiation.
MA1-41017 was used in western blot to study the roles of PPARdelta and gamma in myoblast transdifferentiation
|Holst D,Luquet S,Kristiansen K,Grimaldi PA||Experimental cell research (288:168)||2003|
Thyroid Hormone Stimulation of Autophagy Is Essential for Mitochondrial Biogenesis and Activity in Skeletal Muscle.
MA1-41017 was used in western blot to investigate thyroid hormone-mediated autophagy in skeletal muscle
|Lesmana R,Sinha RA,Singh BK,Zhou J,Ohba K,Wu Y,Yau WW,Bay BH,Yen PM||Endocrinology (157:23)||2016|
Molecular characterization of an EWSR1-POU5F1 fusion associated with a t(6;22) in an undifferentiated soft tissue sarcoma.
MA1-41017 was used in immunohistochemistry to characterize a clinical case of undifferentiated soft tissue sarcoma with chromosomal rearrangement and resultant EWSR1-POU5F1 fusion
|Deng FM,Galvan K,de la Roza G,Zhang S,Souid AK,Stein CK||Cancer genetics (204:423)||2011|
MyoD regulates apoptosis of myoblasts through microRNA-mediated down-regulation of Pax3.
MA1-41017 was used in immunocytochemistry and western blot to study the mechanism by which the MyoD regulates myoblast apoptosis
|Hirai H,Verma M,Watanabe S,Tastad C,Asakura Y,Asakura A||The Journal of cell biology (191:347)||2010|
CD90-positive cells, an additional cell population, produce laminin alpha2 upon transplantation to dy(3k)/dy(3k) mice.
MA1-41017 was used in immunocytochemistry to study the role of CD90-positive cells in producing laminin alpha2 upon transplantation to dy(3k)/dy(3k) mice
|Fukada S,Yamamoto Y,Segawa M,Sakamoto K,Nakajima M,Sato M,Morikawa D,Uezumi A,Miyagoe-Suzuki Y,Takeda S,Tsujikawa K,Yamamoto H||Experimental cell research (314:193)||2008|
Molecular signature of quiescent satellite cells in adult skeletal muscle.
MA1-41017 was used in immunocytochemistry to investigate gene expression in quiescent satellite cells of adult skeletal muscle
|Fukada S,Uezumi A,Ikemoto M,Masuda S,Segawa M,Tanimura N,Yamamoto H,Miyagoe-Suzuki Y,Takeda S||Stem cells (Dayton, Ohio) (25:2448)||2007|