Invitrogen

MYOD Monoclonal Antibody (5.8A)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

6 Published Figures

17 References

View all (37) MYOD antibodies

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Protocols

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MYOD Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of MYOD was performed using 70% confluent log phase differentiated C2C12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MYOD monoclonal antibody (Product # MA1-41017) at 5 µg/mL dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image. Panel e represents undifferentiated C2C12 cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

MYOD Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of human tissues using a the MYOD monoclonal antibody (Product # MA1-41017). A. Rhabdomyosaroma (nuclei are stained), B. Lymphoma (staining is absent)

MYOD Antibody in Western Blot (WB) — Western blot was performed using Anti-MYOD Monoclonal Antibody (Product # MA1-41017) and a 40kDa band corresponding to MYOD was observed across cell lines tested and was observed to increase upon C2C12 differentiated to 3 and 7 days respectively. Modified whole cell extracts (1% SDS) (30 µg lysate) of C2C12 (Lane 1), C2C12 differentiated to myotubes (3 days) (Lane 2) and C2C12 differentiated to myotubes (7 days) (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

MYOD Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells lines tested owing to their inherent genetic constitution. Relative expression of MYOD was observed in C2C12 cells differentiated to myotubes (3 and 7 days) in comparison to undifferentiated C2C12 using Anti-MYOD Monoclonal Antibody (Product # MA1-41017) in Western Blot. {RE}

MYOD Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Fig. 4 Depletion of Hrs affects the expression and localization of the myogenic regulator factors. a Representative Western blotting of shCT and shHrs#3 C2C12 extracts in Pro status (lanes 1 and 7) and at 7, 24, 48, 72, and 96 h of differentiation (lanes 2-6 and 8-12). Membranes were probed with the anti-HRS, -MHC, -Myogenin, -MyoD, and -MEF2A antibodies. GAPDH was used as a loading control. b Quantifications of the myogenin protein level present in experiments like in a . Data are presented as the ratio of myogenin/GAPDH and normalized to the Pro starting point condition. Data represent mean +/- SEM, n = 4 experiments. Significance was assessed using a two-way ANOVA test; ** p < 0.01, **** p < 0.0001. ns not significant. c qRT-PCR of myogenin mRNA realized on mRNAs from shCT and shHrs#3 C2C12 cells collected in Pro and at 7, 24, 48, 72, and 96 h of differentiation. Data are normalized to the Pro starting point condition and presented as mean +/- SEM, n = 4 experiments. Significance was assessed using a two-way ANOVA test; * p < 0.05, **** p < 0.0001. ns not significant. d Quantification of the MyoD protein level present in experiments like in a . Data are presented as the ratio of MyoD/GAPDH signals and normalized to the Pro starting point condition. Data represent mean +/- SEM, n = 5 experiments. Significance was assessed using a two-way ANOVA test. ns not significant. e Representative immunofluorescence images of MyoD in shCT and shHrs#3 C2C12 cells at 24 or 48 h of differ

MYOD Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Published figure using MYOD monoclonal antibody (Product # MA1-41017) in Immunocytochemistry and Immunohistochemistry

Product Details

MA1-41017

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	View 6 publications 6 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Frozen) (IHC (F))	1:10-1:500	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	5 µg/mL	View 6 publications 6 publications
Immunoprecipitation (IP)	1 µg/mL	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	5.8A
Immunogen	Recombinant mouse MyoD.
Conjugate	Unconjugated
Form	Liquid
Concentration	1.0 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2282434

Product Specific Information

The epitope of this antibody was mapped to a region within AA 180-189 of mouse MyoD.

Suggested positive control: Rh-30.

Target Information

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways.

BMC biology

Coudert L,Osseni A,Gangloff YG,Schaeffer L,Leblanc P

MA1-41017 was used in Immunohistochemistry to show for the first time that Hrs/Hgs is a master regulator that modulates myogenesis at different levels through the control of trafficking, signaling, and degradation pathways.

Fri Jul 30 00:00:00 EDT 2021

The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways.

BMC biology

Coudert L,Osseni A,Gangloff YG,Schaeffer L,Leblanc P

Fri Jul 30 00:00:00 EDT 2021

The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways.

BMC biology

Coudert L,Osseni A,Gangloff YG,Schaeffer L,Leblanc P

Fri Jul 30 00:00:00 EDT 2021

The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways.

BMC biology

Coudert L,Osseni A,Gangloff YG,Schaeffer L,Leblanc P

Fri Jul 30 00:00:00 EDT 2021

Ganoderma microsporum immunomodulatory protein, GMI, promotes C2C12 myoblast differentiation in vitro via upregulation of Tid1 and STAT3 acetylation.

PloS one

Teo WH,Lo JF,Fan YN,Huang CY,Huang TF

MA1-41017 was used in Western Blotting to use GMI, immunomodulatory protein of Ganoderma microsporum, as an inducer in C2C12 myoblast differentiation.

Mon Mar 22 00:00:00 EDT 2021

Ganoderma microsporum immunomodulatory protein, GMI, promotes C2C12 myoblast differentiation in vitro via upregulation of Tid1 and STAT3 acetylation.

PloS one

Teo WH,Lo JF,Fan YN,Huang CY,Huang TF

MA1-41017 was used in Western Blotting to use GMI, immunomodulatory protein of Ganoderma microsporum, as an inducer in C2C12 myoblast differentiation.

Mon Mar 22 00:00:00 EDT 2021

Angiotensin-II-induced Muscle Wasting is Mediated by 25-Hydroxycholesterol via GSK3β Signaling Pathway.

EBioMedicine

Shen C,Zhou J,Wang X,Yu XY,Liang C,Liu B,Pan X,Zhao Q,Song JL,Wang J,Bao M,Wu C,Li Y,Song YH

MA141017 was used in immunohistochemistry - frozen section to implicate 25-hydroxycholesterol as an inducer of muscle wasting

Wed Feb 01 00:00:00 EST 2017

Angiotensin-II-induced Muscle Wasting is Mediated by 25-Hydroxycholesterol via GSK3β Signaling Pathway.

EBioMedicine

Shen C,Zhou J,Wang X,Yu XY,Liang C,Liu B,Pan X,Zhao Q,Song JL,Wang J,Bao M,Wu C,Li Y,Song YH

MA141017 was used in immunohistochemistry - frozen section to implicate 25-hydroxycholesterol as an inducer of muscle wasting

Wed Feb 01 00:00:00 EST 2017

Synthetic niche to modulate regenerative potential of MSCs and enhance skeletal muscle regeneration.

Biomaterials

Pumberger M,Qazi TH,Ehrentraut MC,Textor M,Kueper J,Stoltenburg-Didinger G,Winkler T,von Roth P,Reinke S,Borselli C,Perka C,Mooney DJ,Duda GN,Geißler S

MA1-41017 was used in western blot to research modulation of regenerative potential of MSCs and enhancement of skeletal muscle regeneration by a synthetic niche

Mon Aug 01 00:00:00 EDT 2016

Thyroid Hormone Stimulation of Autophagy Is Essential for Mitochondrial Biogenesis and Activity in Skeletal Muscle.

Endocrinology

Lesmana R,Sinha RA,Singh BK,Zhou J,Ohba K,Wu Y,Yau WW,Bay BH,Yen PM

MA1-41017 was used in western blot to investigate thyroid hormone-mediated autophagy in skeletal muscle

Fri Jan 01 00:00:00 EST 2016

The role of TORC1 in muscle development in Drosophila.

Scientific reports

Hatfield I,Harvey I,Yates ER,Redd JR,Reiter LT,Bridges D

MA1-41017 was used in western blot to determine the role of mTORC1 in myogenesis using drosophila

Mon Apr 13 00:00:00 EDT 2015

CD13 regulates anchorage and differentiation of the skeletal muscle satellite stem cell population in ischemic injury.

Stem cells (Dayton, Ohio)

Rahman MM,Ghosh M,Subramani J,Fong GH,Carlson ME,Shapiro LH

Published figure using MYOD monoclonal antibody (Product # MA1-41017) in Immunofluorescence

Sun Jun 01 00:00:00 EDT 2014

CD13 regulates anchorage and differentiation of the skeletal muscle satellite stem cell population in ischemic injury.

Stem cells (Dayton, Ohio)

Rahman MM,Ghosh M,Subramani J,Fong GH,Carlson ME,Shapiro LH

Published figure using MYOD monoclonal antibody (Product # MA1-41017) in Immunofluorescence

Sun Jun 01 00:00:00 EDT 2014

Molecular characterization of an EWSR1-POU5F1 fusion associated with a t(6;22) in an undifferentiated soft tissue sarcoma.

Cancer genetics

Deng FM,Galvan K,de la Roza G,Zhang S,Souid AK,Stein CK

MA1-41017 was used in immunohistochemistry to characterize a clinical case of undifferentiated soft tissue sarcoma with chromosomal rearrangement and resultant EWSR1-POU5F1 fusion

Mon Aug 01 00:00:00 EDT 2011

MyoD regulates apoptosis of myoblasts through microRNA-mediated down-regulation of Pax3.

The Journal of cell biology

Hirai H,Verma M,Watanabe S,Tastad C,Asakura Y,Asakura A

MA1-41017 was used in immunocytochemistry and western blot to study the mechanism by which the MyoD regulates myoblast apoptosis

Mon Oct 18 00:00:00 EDT 2010

CD90-positive cells, an additional cell population, produce laminin alpha2 upon transplantation to dy(3k)/dy(3k) mice.

Experimental cell research

Fukada S,Yamamoto Y,Segawa M,Sakamoto K,Nakajima M,Sato M,Morikawa D,Uezumi A,Miyagoe-Suzuki Y,Takeda S,Tsujikawa K,Yamamoto H

MA1-41017 was used in immunocytochemistry to study the role of CD90-positive cells in producing laminin alpha2 upon transplantation to dy(3k)/dy(3k) mice