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Invitrogen

MYOD Monoclonal Antibody (5.8A)

16 Published Figures
27 References
View all (47) MYOD antibodies

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Product Details

MA5-12902

Applications
Tested Dilution
Publications

Western Blot (WB)

2-3 µg/mL
View 6 publications 6 publications

Immunohistochemistry (IHC)

-
View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2-3 µg/mL
View 14 publications 14 publications

Immunoprecipitation (IP)

2 µg/mL
-

Immunomicroscopy (IM)

Assay-dependent
-
Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat

Published species

Bovine, Chicken, Horse, Human, Mouse, Zebrafish

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

5.8A

Immunogen

Recombinant mouse MyoD1 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982953

Product Specific Information

MA5-12902 targets MyoD1 in IF, IM, and IP applications and shows reactivity with Chicken, Human, mouse, and Rat samples.

The MA5-12902 immunogen is recombinant mouse MyoD1 protein.

Target Information

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: class C basic helix-loop-helix protein 1; myf-3; MyoD; MyoD1; MYOD1 homolog; myogenic factor 1; myogenic factor 3; unnamed protein product

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Gene Aliases: bHLHc1; CMYO17; CMYP17; MYF3; MYOD; Myod-1; MYODRIF; PUM

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UniProt ID: (Chicken) P16075, (Human) P15172, (Rat) Q02346, (Mouse) P10085

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Entrez Gene ID: (Chicken) 374048, (Human) 4654, (Rat) 337868, (Mouse) 17927

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin DNA binding identical protein binding bHLH transcription factor binding protein dimerization activity E-box binding promoter-specific chromatin binding RNA polymerase II regulatory region sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding ligand-dependent nuclear receptor binding enzyme binding ubiquitin protein ligase binding protein homodimerization activity sequence-specific DNA binding RNA polymerase II sequence-specific DNA binding transcription factor binding sequence-specific double-stranded DNA binding basic helix-loop-helix transcription factor
Process(es)
muscle organ development skeletal muscle cell differentiation muscle cell fate commitment histone H3 acetylation histone H4 acetylation positive regulation of myoblast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of skeletal muscle fiber development cellular response to estradiol stimulus positive regulation of myoblast fusion positive regulation of snRNA transcription by RNA polymerase II regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter skeletal muscle tissue development tissue development regulation of gene expression myotube cell development muscle cell differentiation protein unfolding skeletal muscle tissue regeneration regulation of RNA splicing animal organ development positive regulation of muscle cell differentiation regulation of alternative mRNA splicing, via spliceosome myoblast fate determination myoblast fusion cellular response to starvation skeletal muscle atrophy myotube differentiation myotube differentiation involved in skeletal muscle regeneration response to muscle stretch positive regulation of skeletal muscle tissue regeneration skeletal muscle fiber adaptation myoblast differentiation positive regulation of transcription, DNA-templated skeletal muscle fiber development striated muscle cell differentiation cellular response to tumor necrosis factor cellular response to glucocorticoid stimulus cellular response to oxygen levels negative regulation of myoblast proliferation cardiac muscle cell differentiation
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