Western blot analysis of Macro H2A.2 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human H2AFY2-transfected 293T cells. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Macro H2A.2 polyclonal antibody (Product # PA5-34877) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 197 of Macro H2A.2 (Uniprot ID#Q9P0M6)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The PA5-34877 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 197 of Macro H2A.2 (Uniprot ID#Q9P0M6).
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.