|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Human spleen cell homogenate, depleted of lymphocytes and erythrocytes.|
|Storage buffer||tissue culture supernatant with 0.7% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-82669 recognizes an antigen expressed by human macrophages monocytes and histiocytes, but does not stain interdigitating dendritic cells, Langerhans cells, microglial cells or lymphoid cells. Some staining of basophilic granulocytes and of mast cells may be seen. In immunohistochemical studies, the antibody shows partial cross-blocking with some anti-CD68 monoclonal antibodies, suggesting that the epitope may be associated with this antigen.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is spleen.
L1, or Calprotectin molecule, is an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. The antigen is expressed by granulocytes, monoctyes and tissue macrophages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.