Immunohistochemistry analysis of Macrophages/Monocytes Antibody was performed on asian elephant lymph node. To expose target proteins, antigen retrieval was performed by pressure cooker tissues for 10 minutes. Following antigen retrieval, endogenous peroxidases were blocked with 3% hydrogen peroxide for 10 min at room temperature. Tissue slides were washed with deionized water and PBS, and then blocked in goat-serum for 30 min at room temperature. Tissues were probed with a Macrophages/Monocytes Antibody (Product # MA1-80446) diluted 1:200 in goat-serum (upper) or mouse IgG as a negative control (bottom) for 1 hour at 23°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using a biotinylated anti-mouse secondary antibody and avidin-conjugated to horseradish peroxidase, then followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Images were taken at 40X magnification.
|Tested species reactivity||Bovine, Dog, Deer, Horse, Cat, Guinea pig, Goat, Human, Mammal, Non-human primate, Pig, Rabbit, Rat|
|Published species reactivity||Dog, Pig, Rat, Bovine, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:100|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-80446 was successfully to detect Macrophages/Monocytes in IHC application.
This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. A suggested positive control for immunohistochemical applications is human spleen. For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
L1, or Calprotectin molecule, is an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. The antigen is expressed by granulocytes, monoctyes and tissue macrophages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Morphological and physiological retinal degeneration induced by intravenous delivery of vitamin A dimers in rabbits.
MA1-80446 was used in immunohistochemistry - paraffin section to determine the induction by intravenous delivery of vitamin A dimers in rabbits due to physiological and morphological retinal degeneration
|Penn J,Mihai DM,Washington I||Disease models and mechanisms (8:131)||2015|
Polymeric embolization coil of bilayered polyvinyl alcohol strand for therapeutic vascular occlusion: a feasibility study in canine experimental vascular models.
MA1-80446 was used in immunohistochemistry to test polyvinyl alcohol polymer coil as an endovascular embolic agent in a canine vascular model
|Jung SC,Choi SH,Cho HR,Lee TH,Kim TY,Jeong W,Rhee K,Jho JY,Kim JH,Han MH||Journal of vascular and interventional radiology : JVIR (26:117)||2015|
Short-term safety and efficacy of the biodegradable iron stent in mini-swine coronary arteries.
MA1-80446 was used in immunohistochemistry to use a mini-swine model to study the safety and efficacy of a coronary artery biodegradable iron stent
|Wu C,Qiu H,Hu XY,Ruan YM,Tian Y,Chu Y,Xu XL,Xu L,Tang Y,Gao RL||Chinese medical journal (126:4752)||2013|
Xenotransplantation of human unrestricted somatic stem cells in a pig model of acute myocardial infarction.
MA1-80446 was used in immunohistochemistry to study whether xenotransplantation with human CD34-/CD45- unrestricted somatic stem cells is of therapeutic benefit in a porcine model of acute myocardial infarction
|Gahremanpour A,Vela D,Zheng Y,Silva GV,Fodor W,Cardoso CO,Baimbridge F,Fernandes MR,Buja LM,Perin EC||Xenotransplantation (20:110)||2013|
Transplantation with autologous mesenchymal stem cells after acute myocardial infarction evaluated by magnetic resonance imaging: an experimental study.
MA1-80446 was used in immunohistochemistry to study the effects of transplantation with autologous mesenchymal stem cells in postinfarction swine hearts
|Lu M,Zhao S,Liu Q,Jiang S,Song P,Qian H,Zhang Y,Ling J,Yan C,Cheng H,Ma N,Zhao H,Liu Y||Journal of thoracic imaging (27:125)||2012|
Gene expression profile of vascular endothelial growth factor (VEGF) and its receptors in various cell types of the canine lymph node using laser capture microdissection (LCM).
MA1-80446 was used in immunohistochemistry to study the expression of VEGF and its receptors in a range of cell types in normal canine lymph nodes.
|Jais A,Klein D,Wolfesberger B,Walter I||Veterinary immunology and immunopathology (140:207)||2011|
The performance of photooxidatively crosslinked acellular bovine jugular vein conduits in the reconstruction of connections between pulmonary arteries and right ventricles.
MA1-80446 was used in immunohistochemistry to evaluate photooxidatively crosslinked acellular bovine jugular vein conduits in vascular reconstruction
|Lü WD,Zhang M,Wu ZS,Hu TH,Xu ZJ,Liu W,Hu YR||Biomaterials (31:2934)||2010|
Chronic intake of a high-cholesterol diet resulted in hepatic steatosis, focal nodular hyperplasia and fibrosis in non-obese mice.
MA1-80446 was used in immunohistochemistry to investigate the influence of high-cholesterol food on liver function in normal mice
|Sumiyoshi M,Sakanaka M,Kimura Y||The British journal of nutrition (103:378)||2010|
Characterization of a preclinical model of chronic ischemic wound.
MA1-80446 was used in immunohistochemistry to develop and evaluate a preclinical porcine model of chronic ischemic wound
|Roy S,Biswas S,Khanna S,Gordillo G,Bergdall V,Green J,Marsh CB,Gould LJ,Sen CK||Physiological genomics (37:211)||2009|
Role of cardiopulmonary bypass and arrested heart status in the early cell distribution after intracoronary infusion of bone marrow stromal cells.
MA1-80446 was used in immunohistochemistry to study the distribution of cells following intracoronary infusion of bone marrow stromal cells
|Song P,Zhang H,Lu MJ,Li J,Liu XW,Wei YJ,Hu SS||The Journal of surgical research (153:66)||2009|
Injection of bone marrow mesenchymal stem cells in the borderline area of infarcted myocardium: heart status and cell distribution.
MA1-80446 was used in immunohistochemistry to study the distribution of bone marrow mesenchymal stem cells following injection into infarcted myocardium
|Zhang H,Song P,Tang Y,Zhang XL,Zhao SH,Wei YJ,Hu SS||The Journal of thoracic and cardiovascular surgery (134:1234)||2007|
Histopathology of spleen allograft rejection in miniature swine.
MA1-80446 was used in immunohistochemistry to study the histopathology of spleen allograft rejection in miniature swine
|Dor FJ,Gollackner B,Kuwaki K,Ko DS,Cooper DK,Houser SL||International journal of experimental pathology (86:57)||2005|
Insulin increases resistance to burn wound infection-associated sepsis.
MA1-80446 was used in flow cytometry to investigate the therapeutic efficacy of insulin on the treatment of sepsis
|Gauglitz GG,Toliver-Kinsky TE,Williams FN,Song J,Cui W,Herndon DN,Jeschke MG||Critical care medicine (38:202)||2010|