|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Myeloid precursor cells.|
|Storage buffer||PBS with 0.1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-40079 detects ER-MP58, an antigen present on all mouse macrophage colony stimulating factor (M-CSF) responsive cells.
MA1-40079 has been successfully used in immunohistochemistry (frozen) and flow cytometry applications. Typical starting dilution is 1:50.
The monoclonal antibody ER-MP58 recognizes an antigen present on all mouse macrophage colonystimulating factor (M-CSF) responsive cells in the bone marrow, including the earliest colony-forming myeloid progenitors, as well as by the majority of other myeloid precursors. The antigen is detected on a broad development range of macrophage precursor cells ut to the monocytic level, but also on grnaulocytes. ERxpression is rapidly lost upon maturation beyond the monocytic stage. The antigen disappears in the course of macrophage differentiation. Furthermore the antigen is clearly different from commenly used myeloid markers as Mac-1, F4/80, and Gr-1. The monoclonal antibody ER-MP58 is very useful for the identification of mouse myeloid hemopoietic islands in various organs, and for embryonal studies. Cells committed to the myeloid lineage can be separated from progenitor cells with other differentiation capacities by means of multiparameter cell sorting using monoclonal antibody ER-MP58 in combination with monoclonal antibody ER-MP12 and ER-MP-20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.