|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human Mad2L1|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||5 ug/ml|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-5833 detects Mad2 in human samples.
MA1-5833 has been successfully used in Western blot, immunoprecipitation, and immunohistochemistry procedures on formalin-fixed tissues. MA1-5833 detects a band of approximately 25 kDa.
The MA1-5833 immunogen is recombinant human Mad2L1.
Store at 4°C for 1 month or -20°C for longer periods.
The spindle checkpoint complex is composed of Mad1, Mad2, and Mad3. Mad2 provides feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Researchers have found that deletion of a single Mad2 allele results in a defective mitotic checkpoint; Mad2+/-mice develop lung tumors at high rates after long latencies, implicating defects in the mitotic checkpoint of tumorigenesis. Mad2 is an important target in cancer research because it may sensitize cells to existing chemotherapeutics and its expression may be an indicator of cellular sensitivity to chemotharpy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.