|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Proteins precipitated with AER317 from the phosphocellulose column flow through of extract of human breast cancer MCF-7 cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Gel Shift (GS)||1mg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-100|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13868 targets Major Vault Protein in FACS, GS, IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.
The MA5-13868 immunogen is proteins precipitated with AER317 from the phosphocellulose column flow through of extract of human breast cancer MCF-7 cells.
Major vault protein (MVP) is identical to lung-resistance related protein (LRP). Its shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP. Reportedly, the lung-resistance related protein (LRP) is identical to human MVP. LRP is overexpressed in many neoplastic tissues and cell lines indicating a poor response to chemotherapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MVP expression in the prediction of clinical outcome of locally advanced oral squamous cell carcinoma patients treated with radiotherapy.
MA5-13868 was used in immunohistochemistry to study the role and prognostic value of Major Vault Protein in locally advanced oral squamous cell carcinoma
|Henríquez-Hernández LA,Moreno M,Rey A,Lloret M,Lara PC||Radiation oncology (London, England) (7:null)||2012|
Involvement of extracellular signal-regulated kinase/mitogen-activated protein kinase pathway in multidrug resistance induced by HBx in hepatoma cell line.
MA5-13868 was used in western blot to investigate the role of HBx protein on the occurrence of multidrug resistance in hepatoma cell line
|Guan J,Chen XP,Zhu H,Luo SF,Cao B,Ding L||World journal of gastroenterology (10:3522)||2004|