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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:1,000 | - |
ELISA (ELISA) |
1:5,000-1:20,000 | - |
Immunoprecipitation (IP) |
1:500-1:5,000 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Pig |
Host/Isotype |
Sheep / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Malate Dehydrogenase [Pig Heart] |
Conjugate |
Biotin |
Form |
Lyophilized |
Concentration |
1 mg/mL |
Purification |
Ion-exchange chromatography |
Storage buffer |
0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl |
Contains |
0.01% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Malate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other sources is unknown.
MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Aromatic alpha-keto acid reductase; Cytosolic malate dehydrogenase; KAR; malate dehydrogenase (EC 1.1.1.37); malate dehydrogenase 1, NAD (soluble); Malate dehydrogenase, cytoplasmic
Gene Aliases: MDH; MDH1
UniProt ID: (Pig) P11708
Entrez Gene ID: (Pig) 396894
Molecular Function:
dehydrogenase
oxidoreductase
metabolite interconversion enzyme
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