Rockland

Malate Dehydrogenase Polyclonal Antibody, Biotin

Product Details

Applications

Tested Dilution

Publications

ELISA (ELISA)

1:5,000-1:20,000

Immunoprecipitation (IP)

1:500-1:5,000

Western Blot (WB)

1:1,000

Product Specifications

Species Reactivity

Pig

Host / Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Malate Dehydrogenase [Pig Heart]

Conjugate

Biotin

Form

Lyophilized

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Malate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other sources is unknown.

Target Information

MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.