Immunofluorescent analysis of CI-MPR (green) showing staining in the cytoplasm and nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CI-MPR polyclonal antibody (Product # PA3-850) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Bovine, Human, Mouse, Non-human primate|
|Published species reactivity||Non-human primate, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||300 kDa cation-independent receptor purified from adult bovine liver tissue.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:1000|
|Western Blot (WB)||1:2000-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 2 publications below|
PA3-850 detects cation-independent mannose-6-receptor(CI-MPR) in human, bovine, mouse and non-human primate cells.
PA3-850 has been successfully used in ICC/IF, Dot blot and Western blotting procedures. By Western blot, it detects a 300 kDa protein representing CI-MPR under non-reducing conditions.
The PA3-850 immunogen is a 300 kDa cation-independent receptor purified from adult Bovine liver tissue.
The cation-independent mannose-6-phosphate receptor (CI-MPR) is a multifunctional, type I integral membrane glycoprotein, acting as both an intracellular protein carrier and a cell surface signaling molecule. Functionally, CI-MPR binds newly synthesized mannose-6-phosphate and transports it to the lysosome in mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Expression of insulin-like growth factor 2 receptor in corneal keratocytes during differentiation and in response to wound healing.
PA3-850 was used in immunocytochemistry, immunohistochemistry - paraffin section, and western blot to study corneal keratocytes and expression of insulin-like growth factor 2 receptor during wound healing response and differentiation
|Bohnsack RN,Warejcka DJ,Wang L,Gillespie SR,Bernstein AM,Twining SS,Dahms NM||Investigative ophthalmology and visual science (55:7697)||2014|
Identification of novel murine- and human-specific RPGRIP1 splice variants with distinct expression profiles and subcellular localization.
PA3-850 was used in immunocytochemistry to investigate the expression profiles of RPGRIP1 isoforms and their respective cellular distribution
|Lu X,Ferreira PA||Investigative ophthalmology and visual science (46:1882)||2005|