|Tested species reactivity||Dog, Cat, Human, Non-human primate|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human mast cell tryptase purified from human lung tissues|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2000|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11711 targets Mast Cell Tryptase in IHC (P) and WB applications and shows reactivity with Canine, Feline, Human, and Non-human primate samples.
The MA5-11711 immunogen is human mast cell tryptase purified from human lung tissues.
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. A substantial quantity of tryptase is estimated to be found in mast cells of skin and lung and suggestes this enzyme plays a major role in mast cell mediated events. In vitro studies indicate tryptase can cleave C3 to form C3a anaphylatoxin, inactivate fibrinogen as a coaguable substrate for thrombin and activate latent collagenase. Human mast cell tryptase has considerable potential as a marker for mast cell activation as well as being a mediator of inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The role of macrophage migration inhibitory factor in mast cell-stimulated fibroblast proliferation and collagen production.
MA5-11711 was used in immunocytochemistry to develop a human mast cell culture system and study the effect of Macrophage Migration Inhibitory Factor supplementation
|Ningyan G,Xu Y,Hongfei S,Jingjing C,Min C||PloS one (10:null)||2015|
Methods for the study of mast cells in cancer.
MA5-11711 was used in immunohistochemistry to develop methods to study the role of mast cells in cancer
|Blatner NR,Tsai F,Khazaie K||Methods in molecular biology (Clifton, N.J.) (1220:443)||2014|
Activation of cutaneous immune responses in complex regional pain syndrome.
MA5-11711 was used in immunohistochemistry to study cell proliferation and the cellular source of inflammatory mediators in complex regional pain syndrome using a rat fracture model
|Birklein F,Drummond PD,Li W,Schlereth T,Albrecht N,Finch PM,Dawson LF,Clark JD,Kingery WS||The journal of pain : official journal of the American Pain Society (15:485)||2014|
Regional Differences in Chronic Stress-induced Alterations in Mast Cell and Protease-activated Receptor-2-positive Cell Numbers in the Colon of Ws/Ws Rats.
MA5-11711 was used in immunohistochemistry to study the effects of chronic psychological stress on rat colonic mucosal mast cell and PAR-2-positive cell numbers
|Kim YS,Lee MY,Ryu HS,Choi ES,Oh JT,Yun KJ,Choi SC||Journal of neurogastroenterology and motility (20:54)||2014|
The mandibular ridge oral mucosa model of stromal influences on the endothelial tip cells: an immunohistochemical and TEM study.
MA5-11711 was used in immunohistochemistry to study the morphology of oral mucosa endothelial tip cells and factors influencing the healing process
|Rusu MC,Didilescu AC,St¿nescu R,Pop F,M¿noiu VM,Jianu AM,Vâlcu M||Anatomical record (Hoboken, N.J. : 2007) (296:350)||2013|
Histamine induces the neuronal hypertrophy and increases the mast cell density in gastrointestinal tract.
MA5-11711 was used in immunohistochemistry to study the effects of histamine on gastrointestinal tract mast cell abundance and neuronal hypertrophy
|Keles N,Yavuz Arican R,Coskun M,Elpek GO||Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie (64:713)||2012|
Pressure overload leads to an increase of cardiac resident stem cells.
MA5-11711 was used in immunohistochemistry to study the increase in cardiac resident stem cells observed in human hearts subjected to pressure overload
|Rupp S,Bauer J,von Gerlach S,Fichtlscherer S,Zeiher AM,Dimmeler S,Schranz D||Basic research in cardiology (107:null)||2012|
Soluble mediators released from PI-IBS patients' colon induced alteration of mast cell: involvement of reactive oxygen species.
MA5-11711 was used in immunohistochemistry to study the role of reactive oxygen species in modulating peritoneal mast cell activation by soluble factors in post-infective irritable bowel syndrome
|Han W,Lu X,Jia X,Zhou T,Guo C||Digestive diseases and sciences (57:311)||2012|
Increased expression of CC chemokine ligand 18 in patients with chronic rhinosinusitis with nasal polyps.
MA5-11711 was used in immunohistochemistry to study the expression of CCL18 in patients with chronic rhinosinusitis with or without polyps
|Peterson S,Poposki JA,Nagarkar DR,Chustz RT,Peters AT,Suh LA,Carter R,Norton J,Harris KE,Grammer LC,Tan BK,Chandra RK,Conley DB,Kern RC,Schleimer RP,Kato A||The Journal of allergy and clinical immunology (129:119)||2012|
Immediate anaphylactic death following antibiotics injection: splenic eosinophilia easily revealed by pagoda red stain.
MA5-11711 was used in immunohistochemistry to evaluate pagoda red stain for the forensic analysis of anaphylactic death
|Trani N,Bonetti LR,Gualandri G,Barbolini G||Forensic science international (181:21)||2008|
Identification of mast cells and mast cell subpopulations.
MA5-11711 was used in immunohistochemistry to discuss methods for the detection of mast cells and mast cell subpopulations
|Buckley M,Walls AF||Methods in molecular medicine (138:285)||2008|
Postmortem diagnosis of fatal anaphylaxis during intravenous administration of therapeutic and diagnostic agents: evaluation of clinical laboratory parameters and immunohistochemistry in three cases.
MA5-11711 was used in immunohistochemistry to report on the postmortem diagnosis of fatal anaphylaxis during intravenous administration of therapeutic and diagnostic agents
|Osawa M,Satoh F,Horiuchi H,Tian W,Kugota N,Hasegawa I||Legal medicine (Tokyo, Japan) (10:143)||2008|
Interstitial Cajal-like cells in rat mesentery: an ultrastructural and immunohistochemical approach.
MA5-11711 was used in immunohistochemistry to characterize the interstitial Cajal-like cells in rat mesentery
|Hinescu ME,Popescu LM,Gherghiceanu M,Faussone-Pellegrini MS||Journal of cellular and molecular medicine (12:260)||2008|
Elastofibroma: a clinicopathologic and immunohistochemical study of seven cases and literature review.
MA5-11711 was used in immunohistochemistry to report on seven cases of elastofibroma
|Gun BD,Bahadir B,Behzatoglu K,Gun MO,Ozdamar SO||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (115:115)||2007|
Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC).
MA5-11711 was used in immunohistochemistry to investigate the presence and role of interstitial Cajal-like cells in atrial myocardium
|Popescu LM,Gherghiceanu M,Hinescu ME,Cretoiu D,Ceafalan L,Regalia T,Popescu AC,Ardeleanu C,Mandache E||Journal of cellular and molecular medicine (10:429)||2006|
Immune-reconstituted influenza virosome containing CD40L gene enhances the immunological and protective activity of a carcinoembryonic antigen anticancer vaccine.
MA5-11711 was used in immunohistochemistry to investigate the influence of CD40L on the efficiency of specific anticancer vaccine
|Cusi MG,Del Vecchio MT,Terrosi C,Savellini GG,Di Genova G,La Placa M,Fallarino F,Moser C,Cardone C,Giorgi G,Francini G,Correale P||Journal of immunology (Baltimore, Md. : 1950) (174:7210)||2005|