|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human Mcl-1 protein corresponding to aa 1-327|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg/mg protein lysate|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13929 targets Mcl-1 in immunoprecipitation and Western blot applications and shows reactivity with Human samples.
The MA5-13929 immunogen is recombinant human Mcl-1 protein corresponding to aa 1-327.
Mcl-1 (Myeloid cell leukemia-1) is a member of the bcl-2 family. The carboxy terminal of Mcl-1 and bcl-2 share significant sequence homology. Expression of Mcl-1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g. ionizing radiation, ultraviolet radiation, and alkylating drugs) along with increases in GADD45 and Bax and a decrease in bcl-2. Enhanced expression of Mcl-1, prominently associated with mitochondria, complements the continued expression of bcl-2 in ML-1 cells undergoing differentiation. Like bcl-2, Mcl-1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification of novel target proteins in sebaceous gland carcinoma.
MA5-13929 was used in immunohistochemistry to identify novel therapeutic target proteins in sebaceous gland carcinoma
|Erovic BM,Al Habeeb A,Harris L,Goldstein DP,Kim D,Ghazarian D,Irish JC||Head and neck (35:642)||2013|
Vaccinia virus F1L interacts with Bak using highly divergent Bcl-2 homology domains and replaces the function of Mcl-1.
MA5-13929 was used in immunohistochemistry to study the role of highly divergent viral Bcl-2 homology domains in the interaction of vaccinia virus F1L with Bak
|Campbell S,Hazes B,Kvansakul M,Colman P,Barry M||The Journal of biological chemistry (285:4695)||2010|
Stat3 activation in human endometrial and cervical cancers.
MA5-13929 was used in immunohistochemistry to investigate the activation of Stat3 in human endometrial and cervical cancers
|Chen CL,Hsieh FC,Lieblein JC,Brown J,Chan C,Wallace JA,Cheng G,Hall BM,Lin J||British journal of cancer (96:591)||2007|
Short nucleotide polymorphic insertions in the MCL-1 promoter affect gene expression.
MA5-13929 was used in western blot to study the effect on gene expression of short nucleotide polymorphic insertions in the MCL-1 promoter
|Saxena A,Moshynska OV,Moshynskyy ID,Neuls ED,Qureshi T,Bosch M,Voralia M,Bonham K||Cancer letters (251:114)||2007|
Noxa up-regulation and Mcl-1 cleavage are associated to apoptosis induction by bortezomib in multiple myeloma.
MA5-13929 was used in western blot to study the mechanism by which bortezomib induces apoptosis in multiple myeloma
|Gomez-Bougie P,Wuillème-Toumi S,Ménoret E,Trichet V,Robillard N,Philippe M,Bataille R,Amiot M||Cancer research (67:5418)||2007|