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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human MART-1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is melanoma.
Current literature suggests that MART-1 is indispensable for Pmel17 function and thus plays an important role in regulating mammalian pigmentation and that Melan-A is very useful in distinguishing between desmoplastic melanoma and sclerotic nevi.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
antigen LB39-AA; antigen SK29-AA; Melan-A; melanoma antigen recognized by T-cells 1; MLANA; protein Melan-A
MART-1; MART1; RP11-546N22.1