|Tested species reactivity||Human|
|Published species reactivity||Bovine, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Extract of pigmented melanoma metastases from lymph nodes|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:40-1:80|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 9 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Flow Cytometry (Flow)||See 2 publications below|
|Western Blot (WB)||See 5 publications below|
|Immunohistochemistry (IHC)||See 14 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
MA5-13232 targets Melanoma (gp100) in IHC (P) and WB applications and shows reactivity with Human samples.
The MA5-13232 immunogen is extract of pigmented melanoma metastases from lymph nodes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BLOC-1 and BLOC-3 regulate VAMP7 cycling to and from melanosomes via distinct tubular transport carriers.
MA5-13232 was used in immunocytochemistry to propose that VAMP7 mediates fusion of BLOC-1-dependent transport carriers with melanosomes
|Dennis MK,Delevoye C,Acosta-Ruiz A,Hurbain I,Romao M,Hesketh GG,Goff PS,Sviderskaya EV,Bennett DC,Luzio JP,Galli T,Owen DJ,Raposo G,Marks MS||The Journal of cell biology (214:293)||2016|
Differential recognition of a dileucine-based sorting signal by AP-1 and AP-3 reveals a requirement for both BLOC-1 and AP-3 in delivery of OCA2 to melanosomes.
MA5-13232 was used in immunocytochemistry to study the delivery of OCA2 to melanosomes and the distinct roles played by AP-1 and AP-3
|Sitaram A,Dennis MK,Chaudhuri R,De Jesus-Rojas W,Tenza D,Setty SR,Wood CS,Sviderskaya EV,Bennett DC,Raposo G,Bonifacino JS,Marks MS||Molecular biology of the cell (23:3178)||2012|
Cellular and clinical report of new Griscelli syndrome type III cases.
MA5-13232 was used in immunocytochemistry to report on new cases of Griscelli syndrome type III
|Westbroek W,Klar A,Cullinane AR,Ziegler SG,Hurvitz H,Ganem A,Wilson K,Dorward H,Huizing M,Tamimi H,Vainshtein I,Berkun Y,Lavie M,Gahl WA,Anikster Y||Pigment cell and melanoma research (25:47)||2012|
|Not Applicable||Not Cited||
Proprotein convertases process Pmel17 during secretion.
MA5-13232 was used in immunocytochemistry and western blot to study Pmel17 processing in melanocytes
|Leonhardt RM,Vigneron N,Rahner C,Cresswell P||The Journal of biological chemistry (286:9321)||2011|
Endoscopic ultrasound-guided fine-needle aspiration of intrathoracic and intra-abdominal spindle cell and mesenchymal lesions.
MA5-13232 was used in immunocytochemistry to confirm the diagnostic value of endoscopic ultrasound-guided fine-needle aspiration
|Bean SM,Baker A,Eloubeidi M,Eltoum I,Jhala N,Crowe R,Jhala D,Chhieng DC||Cancer cytopathology (119:37)||2011|
Clinical and cellular characterisation of Hermansky-Pudlak syndrome type 6.
MA5-13232 was used in immunocytochemistry to study the clinical and cellular characteristics of Hermansky-Pudlak syndrome subtype 6
|Huizing M,Pederson B,Hess RA,Griffin A,Helip-Wooley A,Westbroek W,Dorward H,O'Brien KJ,Golas G,Tsilou E,White JG,Gahl WA||Journal of medical genetics (46:803)||2009|
A novel missense mutation (G43S) in the switch I region of Rab27A causing Griscelli syndrome.
MA5-13232 was used in immunocytochemistry to investigate the role of Rab27A specific mutation in Griscelli syndrome
|Westbroek W,Tuchman M,Tinloy B,De Wever O,Vilboux T,Hertz JM,Hasle H,Heilmann C,Helip-Wooley A,Kleta R,Gahl WA||Molecular genetics and metabolism (94:248)||2008|
Improper trafficking of melanocyte-specific proteins in Hermansky-Pudlak syndrome type-5.
MA5-13232 was used in immunocytochemistry to study the trafficking of melanocyte-specific proteins in Hermansky-Pudlak syndrome type-5 melanocytes
|Helip-Wooley A,Westbroek W,Dorward HM,Koshoffer A,Huizing M,Boissy RE,Gahl WA||The Journal of investigative dermatology (127:1471)||2007|
Proprotein convertase cleavage liberates a fibrillogenic fragment of a resident glycoprotein to initiate melanosome biogenesis.
MA5-13232 was used in immunocytochemistry to investigate the role of proprotein convertase cleavage in melanosome biogenesis
|Berson JF,Theos AC,Harper DC,Tenza D,Raposo G,Marks MS||The Journal of cell biology (161:521)||2003|
A challenging case of ocular melanoma.
MA5-13232 was used in immunohistochemistry to present a case of highly aggressive ocular melanoma
|Costache M,Dumitru AV,P¿tra¿cu OM,Popa-Cherecheanu DA,B¿dil¿ P,Miu JC,Procop A,Popa M,Tampa M¿,Sajin M,Simionescu O,Cîrstoiu MM||Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (56:817)||2015|
BLOC-2 targets recycling endosomal tubules to melanosomes for cargo delivery.
MA5-13232 was used in flow cytometry to investigate the role of BLOC-2 in endosomal tubular transport in melanosomes
|Dennis MK,Mantegazza AR,Snir OL,Tenza D,Acosta-Ruiz A,Delevoye C,Zorger R,Sitaram A,de Jesus-Rojas W,Ravichandran K,Rux J,Sviderskaya EV,Bennett DC,Raposo G,Marks MS,Setty SR||The Journal of cell biology (209:563)||2015|
Antigenic profiling of glioma cells to generate allogeneic vaccines or dendritic cell-based therapeutics.
MA5-13232 was used in flow cytometry to examine the allogenic glioma cells for the generation of therapeutic vaccines or cellular therapy
|Zhang JG,Eguchi J,Kruse CA,Gomez GG,Fakhrai H,Schroter S,Ma W,Hoa N,Minev B,Delgado C,Wepsic HT,Okada H,Jadus MR||Clinical cancer research : an official journal of the American Association for Cancer Research (13:566)||2007|
Absence of recognition of common melanocytic antigens by T cells isolated from the cerebrospinal fluid of a Vogt-Koyanagi-Harada patient.
MA5-13232 was used in western blot to identify antigens recognized by autoreactive CD4 T lymphocytes isolated from a Vogt-Koyanagi-Harada patient who did not express HLA-DRB1*04:05
|Abad S,Wieërs G,Colau D,Wildmann C,Delair E,Dhote R,Brézin AP,Kawakami Y,Coulie PG,van der Bruggen P||Molecular vision (20:956)||2014|
Mutations in or near the transmembrane domain alter PMEL amyloid formation from functional to pathogenic.
MA5-13232 was used in western blot to investigate the role of PMEL mutations in physiological and pathological amyloid fibril formation
|Watt B,Tenza D,Lemmon MA,Kerje S,Raposo G,Andersson L,Marks MS||PLoS genetics (7:null)||2011|
Cell-specific ATP7A transport sustains copper-dependent tyrosinase activity in melanosomes.
MA5-13232 was used in western blot to investigate the role of cell-specific ATP7A transport in copper-dependent tyrosinase activity of melanosomes
|Setty SR,Tenza D,Sviderskaya EV,Bennett DC,Raposo G,Marks MS||Nature (454:1142)||2008|
Melanocytes expressing MC1R polymorphisms associated with red hair color have altered MSH-ligand activated pigmentary responses in coculture with keratinocytes.
MA5-13232 was used in western blot to study the behavior and functional abilities of melanocytes expressing MC1R red hair color variants
|Roberts DW,Newton RA,Leonard JH,Sturm RA||Journal of cellular physiology (215:344)||2008|
Rab7 regulates maturation of melanosomal matrix protein gp100/Pmel17/Silv.
MA5-13232 was used in western blot to study the regulation of the melanosomal matrix protein gp100 by Rab7
|Kawakami A,Sakane F,Imai S,Yasuda S,Kai M,Kanoh H,Jin HY,Hirosaki K,Yamashita T,Fisher DE,Jimbow K||The Journal of investigative dermatology (128:143)||2008|
ASPL-TFE3 translocation in vulvovaginal alveolar soft part sarcoma.
MA5-13232 was used in immunohistochemistry to report on a case of vulvovaginal alveolar soft part sarcoma
|Jabbour MN,Seoud M,Al-Ahmadie H,Abdul-Karim FW,Zaatari GS||International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (33:263)||2014|
Targeting BRAFV600E in an inducible murine model of melanoma.
MA5-13232 was used in immunohistochemistry to study the effects of targeting BRAF(V600E) on melanoma growth in an inducible murine model
|Hooijkaas AI,Gadiot J,van der Valk M,Mooi WJ,Blank CU||The American journal of pathology (181:785)||2012|
Genetic ablation of SOX18 function suppresses tumor lymphangiogenesis and metastasis of melanoma in mice.
MA5-13232 was used in immunohistochemistry to study the role of SOX18 function function in tumor lymphangiogenesis and metastasis of melanoma in mice
|Duong T,Proulx ST,Luciani P,Leroux JC,Detmar M,Koopman P,Francois M||Cancer research (72:3105)||2012|
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
MA5-13232 was used in immunohistochemistry to report on a case of sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression
|Yin WH,Li J,Chan JK||Diagnostic pathology (7:null)||2012|
Effect of PUVA therapy on melanocytes and keratinocytes in non-segmental vitiligo: histopathological, immuno-histochemical and ultrastructural study.
MA5-13232 was used in immunohistochemistry to study the effects of PUVA treatment on melanocytes and keratinocytes from patients with non-segmental vitiligo
|Anbar TS,El-Sawy AE,Attia SK,Barakat MT,Moftah NH,El-Ammawy TS,Abdel-Rahman AT,El-Tonsy MH||Photodermatology, photoimmunology and photomedicine (28:17)||2012|
Primary myoepithelial carcinoma of the larynx: case report and review of the literature.
MA5-13232 was used in immunohistochemistry to report on a case of primary myoepithelial carcinoma of the larynx
|Yu G,Qu G,Kong L,Pan X,Wang W,Lv J||Pathology, research and practice (207:127)||2011|
Microscopic uterine lymphangioleiomyomatosis perivascular epithelioid cell neoplasm: a case report with the earliest manifestation of this enigmatic neoplasm.
MA5-13232 was used in immunohistochemistry to report a clinical case of microscopic lymphangioleiomyomatosis perivascular epithelioid cell neoplasm in uterus
|Clay MR,Gibson P,Lowell J,Cooper K||International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (30:71)||2011|
Cutaneous metastases of malignant melanoma--how difficult can it be?
MA5-13232 was used in immunohistochemistry to investigate the differentiating features between primary melanoma and its cutaneous metastasis
|Zurac S,Andrei R,Petsakos G,Nichita L,Bastian A,Micu G,Gramad¿ E,Popp C,St¿niceanu F,Petrescu S,Negroiu G,Giurc¿neanu D,Chi¿u V||Romanian journal of internal medicine = Revue roumaine de medecine interne (46:375)||2009|
Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity.
MA5-13232 was used in immunohistochemistry to study pulmonary meningothelial-like nodules
|Mukhopadhyay S,El-Zammar OA,Katzenstein AL||The American journal of surgical pathology (33:487)||2009|
Epithelioid smooth muscle tumors of the uterus do not express CD1a: a potential immunohistochemical adjunct in their distinction from uterine perivascular epithelioid cell tumors.
MA5-13232 was used in immunohistochemistry to investigate the level of CD1a in uterine epithelioid smooth muscle tumors
|Fadare O,Liang SX||Annals of diagnostic pathology (12:401)||2008|
Vacuolar-type H(+)-ATPase with the a3 isoform is the proton pump on premature melanosomes.
MA5-13232 was used in immunohistochemistry to identify the proton pump on immature melanosomes as the vacuolar H(+)-ATPase a3 isoform
|Tabata H,Kawamura N,Sun-Wada GH,Wada Y||Cell and tissue research (332:447)||2008|
Melanosis in a Holstein heifer.
MA5-13232 was used in immunohistochemistry to investigate histological features in a Holstein heifer with melanosis
|Oruç E||Journal of veterinary medicine. A, Physiology, pathology, clinical medicine (54:501)||2007|
Characterization of t(6;11)(p21;q12) in a renal-cell carcinoma of an adult patient.
MA5-13232 was used in immunohistochemistry to report a clinical case of t(6;11)(p21;q12) renal-cell carcinoma
|Pecciarini L,Cangi MG,Lo Cunsolo C,Macri' E,Dal Cin E,Martignoni G,Doglioni C||Genes, chromosomes and cancer (46:419)||2007|
Accumulation of low-avidity anti-melanocortin receptor 1 (anti-MC1R) CD8+ T cells in the lesional skin of a patient with melanoma-related depigmentation.
MA5-13232 was used in immunohistochemistry to investigat specific CD8+ tissue-infiltrating T cells in a patient with melanoma
|Wankowicz-Kalinska A,Mailliard RB,Olson K,Graham F,Edington H,Kirkwood JM,Martinek S,Das PK,Storkus WJ||Melanoma research (16:165)||2006|
Cardiac Epithelioid PEComa: Report of Two Cases and Review of the Literature.
MA5-13232 was used in immunohistochemistry - paraffin section to present two cases of epithelioid PEComas
|Niu H,Wang FW,Zhang PJ,Bing Z||Case reports in medicine (2012:null)||2012|
Mannose receptor targeting of tumor antigen pmel17 to human dendritic cells directs anti-melanoma T cell responses via multiple HLA molecules.
MA5-13232 was used in blocking or activating experiment to study the T cell immune response against melanoma elicited through conjugation of melanoma antigen with mannose receptor antibody
|Ramakrishna V,Treml JF,Vitale L,Connolly JE,O'Neill T,Smith PA,Jones CL,He LZ,Goldstein J,Wallace PK,Keler T,Endres MJ||Journal of immunology (Baltimore, Md. : 1950) (172:2845)||2004|