|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat, Hamster, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues K M(1) N S P S E S R V P S S L T Q D P S F(19) of rat melanopsin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||10 ug/ml|
|Immunofluorescence (IF)||10 ug/ml|
|Western Blot (WB)||1-4 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-780 detects mouse and rat melanopsin.
PA1-780 has been successfully used in Western blot, immunofluorescence, and immunocytochemistry procedures. By Western blot, this antibody detects ~53 and ~85 kDa proteins representing overexpressed unglycosylated and glycosylated melanopsin from HEK 293 cells (does not work well on endogenously expressed protein). Immunofluorescence and immunocytochemistry staining of melanopsin in rat retinal ganglion cells (RGCs) can be performed using PA1-780.
PA1-780 immunogen is a synthetic peptide corresponding to residues K M(1) N S P S E S R V P S S L T Q D P S F(19) of rat melanopsin.
Melanopsin is an opsin-like protein found in the retinal ganglion cells of mammals. Melanopsin is believed to be part of a secondary optical system that parallels that of the classic rod and cone system. This second system reports directly to the suprachiasmatic nucleus and is responsible for regulation of circadian rhythms. Melanopsin is believed to be the primary photopigment responsible for the regulation of these circadian rhythms, and Melanopsin knockout mice have been generated which demonstrate decreased capacity to entrain to light and dark cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Age-related changes in photosensitive melanopsin-expressing retinal ganglion cells correlate with circadian rhythm impairments in sighted and blind rats.
PA1-780 was used in immunohistochemistry - paraffin section to study the correlation between circadian rhythm impairments in sighted and blind rats and age-related changes in photosensitive melanopsin-expressing retinal ganglion cells
|Lax P,Esquiva G,Fuentes-Broto L,Segura F,Sánchez-Cano A,Cuenca N,Pinilla I||Chronobiology international (33:374)||2016|
The Dorsal Raphe Nucleus Receives Afferents From Alpha-Like Retinal Ganglion Cells and Intrinsically Photosensitive Retinal Ganglion Cells in the Rat.
PA1-780 was used in immunohistochemistry to study the dorsal raphe nucleus and afferents received by alpha-like retinal ganglion cells and photosensitive retinal ganglion cells in rats
|Li X,Ren C,Huang L,Lin B,Pu M,Pickard GE,So KF||Investigative ophthalmology and visual science (56:8373)||2015|
Retino-retinal projection in juvenile and young adult rats and mice.
PA1-780 was used in immunohistochemistry to assess the presence of ret-ret RGCs in juvenile and young adult rats and mice by using retrograde tracers applied to the contralateral optic nerve
|Nadal-Nicolás FM,Valiente-Soriano FJ,Salinas-Navarro M,Jiménez-López M,Vidal-Sanz M,Agudo-Barriuso M||Experimental eye research (134:47)||2015|
Moniliform deformation of retinal ganglion cells by formaldehyde-based fixatives.
PA1-780 was used in immunohistochemistry to improve formaldehyde-based fixatives to minimize bead formation of retinal ganglion cells.
|Stradleigh TW,Greenberg KP,Partida GJ,Pham A,Ishida AT||The Journal of comparative neurology (523:545)||2015|
Direct retino-raphe projection alters serotonergic tone and affective behavior.
PA1-780 was used in immunohistochemistry to study the neural circuits involved in the modulation of mood by light
|Ren C,Luan L,Wui-Man Lau B,Huang X,Yang J,Zhou Y,Wu X,Gao J,Pickard GE,So KF,Pu M||Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (38:1163)||2013|
Disturbed sleep/wake rhythms and neuronal cell loss in lateral hypothalamus and retina of mice with a spontaneous deletion in the ubiquitin carboxyl-terminal hydrolase L1 gene.
PA1-780 was used in immunohistochemistry to investigate the involvement of ubiquitin carboxyl-terminal hydrolase L1 in the regulation of sleep/wake rhythms and neuronal cell of lateral hypothalamus and retina
|Pfeffer M,Plenzig S,Gispert S,Wada K,Korf HW,Von Gall C||Neurobiology of aging (33:393)||2012|
Quantitative analysis of pupillary light reflex by real-time autofluorescent imaging in a diabetic mouse model.
PA1-780 was used in immunohistochemistry to develop a quantitative method for measuring pupilliary light reflex for early detection of diabetic retinopathy.
|Kumar S,Zhuo L||Experimental eye research (92:164)||2011|
A neuroanatomical and physiological study of the non-image forming visual system of the cone-rod homeobox gene (Crx) knock out mouse.
PA1-780 was used in immunohistochemistry to study the the non-image forming visual system in the cone-rod homeobox gene knockout mouse
|Rovsing L,Rath MF,Lund-Andersen C,Klein DC,Møller M||Brain research (1343:54)||2010|
Immunotoxin-induced ablation of melanopsin retinal ganglion cells in a non-murine mammalian model.
PA1-780 was used in immunohistochemistry to study the effect of immunotoxin on melanopsin retinal ganglion cells
|Ingham ES,Günhan E,Fuller PM,Fuller CA||The Journal of comparative neurology (516:125)||2009|
Retinal neurodegeneration in experimental glaucoma.
PA1-780 was used in immunohistochemistry to study the effects of ocular hypertension in limbar tissues
|Vidal-Sanz M,Valiente-Soriano FJ,Ortín-Martínez A,Nadal-Nicolás FM,Jiménez-López M,Salinas-Navarro M,Alarcón-Martínez L,García-Ayuso D,Avilés-Trigueros M,Agudo-Barriuso M,Villegas-Pérez MP||Progress in brain research (220:1)||2015|
The rat retina has five types of ganglion-cell photoreceptors.
PA1-780 was used in immunocytochemistry to examine the ganglion-cell photoreceptors in rat retina
|Reifler AN,Chervenak AP,Dolikian ME,Benenati BA,Meyers BS,Demertzis ZD,Lynch AM,Li BY,Wachter RD,Abufarha FS,Dulka EA,Pack W,Zhao X,Wong KY||Experimental eye research (130:17)||2015|
Impairment of intrinsically photosensitive retinal ganglion cells associated with late stages of retinal degeneration.
PA1-780 was used in immunocytochemistry to study age-related changes in intrinsically photosensitive retinal ganglion cells in a rat model of autosomal dominant retinitis pigmentosa
|Esquiva G,Lax P,Cuenca N||Investigative ophthalmology and visual science (54:4605)||2013|
Characterization of a new murine retinal cell line (MU-PH1) with glial, progenitor and photoreceptor characteristics.
PA1-780 was used in immunocytochemistry to characterize a mouse Müller-derived cell line that displays glial, progenitor and photoreceptor properties
|Gómez-Vicente V,Flores A,Lax P,Murciano C,Yáñez A,Gil ML,Cuenca N,Gozalbo D,Maneu V||Experimental eye research (110:125)||2013|
Melanopsin ganglion cells use a membrane-associated rhabdomeric phototransduction cascade.
PA1-780 was used in immunocytochemistry to investigate the role of the membrane-associated rhabdomeric phototransduction cascade in melanopsin ganglion cells
|Graham DM,Wong KY,Shapiro P,Frederick C,Pattabiraman K,Berson DM||Journal of neurophysiology (99:2522)||2008|
Differential expression of melanopsin mRNA and protein in Brown Norwegian rats.
PA1-780 was used in western blot to study the regulation of melanopsin mRNA and protein expression in rats subjected to different light/dark cycles
|Hannibal J,Georg B,Fahrenkrug J||Experimental eye research (106:55)||2013|
Restricted expression of Neuroglobin in the mouse retina and co-localization with Melanopsin and Tyrosine Hydroxylase.
PA1-780 was used in western blot to study the role of neuroglobin in the mouse retina
|Hundahl CA,Fahrenkrug J,Luuk H,Hay-Schmidt A,Hannibal J||Biochemical and biophysical research communications (425:100)||2012|
Transfection with pax6 gene of mouse embryonic stem cells and subsequent cell cloning induced retinal neuron progenitors, including retinal ganglion cell-like cells, in vitro.
PA1-780 was used in western blot to investigate the role of pax6 in the differentiation of embryonic stem cells into retinal progenitor cells
|Kayama M,Kurokawa MS,Ueda Y,Ueno H,Kumagai Y,Chiba S,Takada E,Ueno S,Tadokoro M,Suzuki N||Ophthalmic research (43:79)||2010|