|Tested species reactivity||Rat|
|Published species reactivity||Rat, Rodent, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues E(455) Q K S K T P K T K R H L P S L D R R M(474) of rat melanopsin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000|
|Western Blot (WB)||0.1 - 0.2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-781 detects rat melanopsin.
PA1-781 has been successfully used in Western blot, immunofluorescence, and immunocytochemistry procedures. By Western blot, this antibody detects ~53 and ~85 kDa proteins representing overexpressed unglycosylated and glycosylated melanopsin from HEK 293 cells (does not work well on endogenously expressed protein). By immunofluorescence and immunocytochemistry, PA1-781 detects melanopsin from rat retinal ganglion cells (RGCs).
The PA1-781 immunogen is a synthetic peptide corresponding to residues E(455) Q K S K T P K T K R H L P S L D R R M(474) of rat melanopsin.
Melanopsin is an opsin-like protein found in the retinal ganglion cells of mammals. Melanopsin is believed to be part of a secondary optical system that parallels that of the classic rod and cone system. This second system reports directly to the suprachiasmatic nucleus and is responsible for regulation of circadian rhythms. Melanopsin is believed to be the primary photopigment responsible for the regulation of these circadian rhythms, and Melanopsin knockout mice have been generated which demonstrate decreased capacity to entrain to light and dark cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Parallel Inhibition of Dopamine Amacrine Cells and Intrinsically Photosensitive Retinal Ganglion Cells in a Non-Image-Forming Visual Circuit of the Mouse Retina.
PA1-781 was used in immunohistochemistry to compare the inhibition of dopamine amacrine cells and retinal ganglion cells in mouse retina
|Vuong HE,Hardi CN,Barnes S,Brecha NC||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:15955)||2015|
Melanopsin ganglion cells are the most resistant retinal ganglion cell type to axonal injury in the rat retina.
PA1-781 was used in immunohistochemistry to study the greater injury resistance of M1 melanopsin ganglion cells as compared to other ganglion cell types following injury to the optic nerve
|Pérez de Sevilla Müller L,Sargoy A,Rodriguez AR,Brecha NC||PloS one (9:null)||2014|
Intrinsic photosensitive retinal ganglion cells in the diurnal rodent, Arvicanthis ansorgei.
PA1-781 was used in immunohistochemistry to study the properties of the intrnsically photo-sensitive retinal ganglion cells of a diurnal rodent species
|Karnas D,Hicks D,Mordel J,Pévet P,Meissl H||PloS one (8:null)||2013|
Controlling the number of melanopsin-containing retinal ganglion cells by early light exposure.
PA1-781 was used in immunohistochemistry to study the effect of constant light in the first week after birth on the number of retinal ganglion cells expressing melanopsin in a rat model
|Hong J,Zeng Q,Wang H,Kuo DS,Baldridge WH,Wang N||Experimental eye research (111:17)||2013|
Heterogeneity of intrinsically photosensitive retinal ganglion cells in the mouse revealed by molecular phenotyping.
PA1-781 was used in immunohistochemistry to study the heterogeneity of intrinsically photosensitive retinal ganglion cells in the mouse
|Karnas D,Mordel J,Bonnet D,Pévet P,Hicks D,Meissl H||The Journal of comparative neurology (521:912)||2013|
Whole number, distribution and co-expression of brn3 transcription factors in retinal ganglion cells of adult albino and pigmented rats.
PA1-781 was used in immunohistochemistry to study the expression of the three Brn3 factors in the albino and pigmented adult rat
|Nadal-Nicolás FM,Jiménez-López M,Salinas-Navarro M,Sobrado-Calvo P,Alburquerque-Béjar JJ,Vidal-Sanz M,Agudo-Barriuso M||PloS one (7:null)||2012|
Evidence that neurites in human epiretinal membranes express melanopsin, calretinin, rod opsin and neurofilament protein.
PA1-781 was used in immunohistochemistry to investigate the expression of multiple genes in neurites in human epiretinal membranes
|Lesnik Oberstein SY,Lewis GP,Dutra T,Fisher SK||The British journal of ophthalmology (95:266)||2011|
Tracer coupling of intrinsically photosensitive retinal ganglion cells to amacrine cells in the mouse retina.
PA1-781 was used in immunohistochemistry to identify intrinsically photosensitive retinal ganglion cells using melanopsin as a marker
|Müller LP,Do MT,Yau KW,He S,Baldridge WH||The Journal of comparative neurology (518:4813)||2010|
Immunotoxin-induced ablation of melanopsin retinal ganglion cells in a non-murine mammalian model.
PA1-781 was used in immunohistochemistry to study the effect of immunotoxin on melanopsin retinal ganglion cells
|Ingham ES,Günhan E,Fuller PM,Fuller CA||The Journal of comparative neurology (516:125)||2009|
Two types of melanopsin retinal ganglion cell differentially innervate the hypothalamic suprachiasmatic nucleus and the olivary pretectal nucleus.
PA1-781 was used in immunohistochemistry to study the innervation of hypothalamic suprachiasmatic nucleus and the olivary pretectal nucleus by melanopsin retinal ganglion cells
|Baver SB,Pickard GE,Sollars PJ,Pickard GE||The European journal of neuroscience (27:1763)||2008|
Synaptic contact between melanopsin-containing retinal ganglion cells and rod bipolar cells.
PA1-781 was used in immunohistochemistry to study the synaptic contact between melanopsin-containing retinal ganglion cells and rod bipolar cells.
|Østergaard J,Hannibal J,Fahrenkrug J||Investigative ophthalmology and visual science (48:3812)||2007|
Melanopsin-expressing retinal ganglion cells are more injury-resistant in a chronic ocular hypertension model.
PA1-781 was used in immunohistochemistry to investigate the role of melanopsin in the resistance of chronic ocular hypertension.
|Li RS,Chen BY,Tay DK,Chan HH,Pu ML,So KF||Investigative ophthalmology and visual science (47:2951)||2006|
Acute photoreceptor degeneration down-regulates melanopsin expression in adult rat retina.
PA1-781 was used in immunohistochemistry to investigate the effect of acute photoreceptor degeneration by N-methyl-N-nitrosourea on melanopsin expression in adult rats
|Wan J,Zheng H,Hu BY,Xiao HL,She ZJ,Chen ZL,Zhou GM||Neuroscience letters (400:48)||2006|
Localization of melanopsin-immunoreactive cells in the Mongolian gerbil retina.
PA1-781 was used in immunocytochemistry to describe the organization of melanopsin-immunoreactive neurons in the Mongolian gerbil retina
|Jeong MJ,Jeon CJ||Neuroscience research (100:6)||2015|
ß-Arrestin-dependent deactivation of mouse melanopsin.
PA1-781 was used in immunocytochemistry to study light- and phosphorylation- dependent reduction in melanopsin signaling mediated by both beta-arrestin 1 and beta-arrestin 2
|Cameron EG,Robinson PR||PloS one (9:null)||2014|
Phosphorylation of mouse melanopsin by protein kinase A.
PA1-781 was used in immunocytochemistry to study the ability of protein kinase A to phosphorylate melanopsin and the significance for melanopsin signaling
|Blasic JR,Brown RL,Robinson PR||PloS one (7:null)||2012|
Light-dependent phosphorylation of the carboxy tail of mouse melanopsin.
PA1-781 was used in immunocytochemistry to characterize the phosphorylation of mouse melanopsin
|Blasic JR,Lane Brown R,Robinson PR||Cellular and molecular life sciences : CMLS (69:1551)||2012|
||Melanopsin-containing retinal ganglion cells: architecture, projections, and intrinsic photosensitivity.||Hattar S,Liao HW,Takao M,Berson DM,Yau KW||Science (New York, N.Y.) (295:1065)||2002|
N-linked deglycosylated melanopsin retains its responsiveness to light.
PA1-781 was used in western blot to study the effect of deglycosylation on the light-sensitivity of melanopsin in PC12-rMel cell line
|Fahrenkrug J,Falktoft B,Georg B,Rask L||Biochemistry (48:5142)||2009|
Melanopsin changes in neonatal albino rat independent of rods and cones.
PA1-781 was used in western blot to investigate the relationship between melanopsin level and rods/cones
|Hannibal J,Georg B,Fahrenkrug J||Neuroreport (18:81)||2007|
|Rat||1:1,000-2,000||Melanopsin-containing retinal ganglion cells: architecture, projections, and intrinsic photosensitivity.||Hattar S,Liao HW,Takao M,Berson DM,Yau KW||Science (New York, N.Y.) (295:1065)||2002|