|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Spleen lymphocytes from mice fused with OVCAR-3, an ovarian tumor cell line derived from poorly differentiated adenocarcinoma of the ovary.|
|Storage buffer||PBS with 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:40|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
MA1-26527 detects Mesothelin from human and Cynomolgus monkey samples.
MA1-26527 has been successfully used in immunohistochemistry (frozen) procedures.
The MA1-26527 immunogen is spleen lymphocytes from mice fused with OVCAR-3, an ovarian tumor cell line derived from poorly differentiated adenocarcinoma of the ovary.
An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
p53 autoantibodies, cytokine levels and ovarian carcinogenesis.
MA1-26527 was used in ELISA to test if type II ovarian carcinoma, mutation of p53, and plasma levels of particular cytokines are associated with the generation of p53-specific serum autoantibody responses in patients
|Tsai-Turton M,Santillan A,Lu D,Bristow RE,Chan KC,Shih IeM,Roden RB||Gynecologic oncology (114:12)||2009|