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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human mesothelin protein.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is mesothelioma.
Mesothelin is a glycocylphosphotidylianositol-linked cell-surface glycoprotein that is present on the surface of normal mesothelium and is overexpressed in epithelial ovarian cancer and malignant mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CAK1 antigen; megakaryocyte potentiating factor; MPF; pre-pro-megakaryocyte-potentiating factor; soluble MPF mesothelin related protein
MPF; MSLN; SMRP