Western blot analysis of Methyl-Histone H4 (Lys20) in Hela histones using a mono-Methyl-Histone H4 (Lys20) monoclonal antibody (Product # MA5-18067) at various dilutions. Samples were separated by SDS-PAGE and transferred to a nitrocellulose membrane. The membrane was blocked in 3% NFDM-PBST for 1 hr at room temp, incubated at 4°C in 0.5% NFDM-PBST, and detection was performed using an HRP-conjugated Fc-specific anti-mouse secondary antibody.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide of Human Histone H4|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-dependent|
|Dot blot (DB)||Assay-dependent|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is specific for K20 mono-methylated histone H4 and does not cross-react with histone 3 or tri-, di-, or unmethylated histone H4. This antibody is predicted to react with most mammals based on sequence homology.
This target has a predicted molecular weight of 11 kDa. Actual Western blot results show a band at 13 kDa. This can be blocked using mono-methyl K20 Histone H4 peptide.
Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.