Western blot analysis of Methyl-PP2A alpha (Leu309) in two yeast strains using a Methyl-PP2A alpha (Leu309) monoclonal antibody (MA5-18072). Native cultures from yeast deletion strains expressing the HA-tagged PP2AC (PPH21) were subjected to IP against the HA tag: strain PPE1-delta (left) which lacks the PP2A methylesterase (PPE1) and accumulates methylated PPH21, and PPM1-delta which lacks the PP2A methyltransferase and contains no methylated PPH21. The lysates were boiled in Laemmli buffer, separated by SDS-PAGE and transfered to a nitrocellulose membrane. Lane 5 shows a control incubated with an anti-HA tag monoclonal antibody.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A KLH-coupled peptide with the sequence: KLH-C-ßA-RRTPDYFL-OMe|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes the C-terminal methylated form of PP2A (C subunit). The c-terminal sequence, as well as the carboxy-methylation, is 100% conserved in human, mouse and yeast.
Western blot results show a band at 36 kDa, which matches the predicted molecular weight.
The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.