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Methylglyoxal Antibody (MA5-27567) in ICC/IF
Immunofluorescent analysis of Methylglyoxal in human embryonic kidney cells (HEK293). Sample was fixed with 5% formaldehyde (5 min), incubated with Methylglyoxal monoclonal antibody (Product # MA5-27567) using a dilution of 1:50 (30-60 min at RT), and followed by Goat Anti-Mouse Alexa Fluor 488, Phalloidin Alexa Fluor 633 and DAPI secondary antibody at a dilution of 1:1500, 1:250 and 1:50,000 (30-60 min at ... View More
Product Details
MA5-27567
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
9E7
Immunogen
Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2735270
Product Specific Information
A 1:1,000 dilution of MA5-27567 was sufficient for detection of Methylglyoxal in 0.5 µg of Methylglyoxal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.
Target Information
Methylglyoxal, also called pyruvaldehyde or 2-oxopropanal, is the organic compound with the formula CH3C(O)CHO. Gaseous methylglyoxal has two carbonyl groups, an aldehyde and a ketone but in the presence of water, it exists as hydrates and oligomers. Methylglyoxal is a reduced derivative of pyruvic acid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2 oxo propanal; 2-Oxopropanal; Methylglyoxal; Methylglyoxal (MG); MG; MG-modified protein; Pyruvaldehyde
Performance Guarantee
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