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|Tested species reactivity||Dog, Horse, Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||N-terminal fragment of human Mi protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5-1ug/test|
|Gel Shift (GS)||1mg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||2-4 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 21 publications below|
|Immunohistochemistry (IHC)||See 10 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Gel Shift (GS)||See 1 publications below|
MA5-14146 targets MiTF in IHC (paraffin), FACS, GS and WB applications and shows reactivity with Canine, Equine, Human, mouse, and Rat samples.
The MA5-14146 immunogen is n-terminal fragment of human Mi protein.
Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Loss of CITED1, an MITF regulator, drives a phenotype switch in vitro and can predict clinical outcome in primary melanoma tumours.
MA5-14146 was used in western blot to investigate the role of CITED1 in melanoma
|Howlin J,Cirenajwis H,Lettiero B,Staaf J,Lauss M,Saal L,Borg K,Gruvberger-Saal S,Jönsson G||PeerJ (3:null)||2015|
Hydroalcoholic extract of Rhodiola rosea L. (Crassulaceae) and its hydrolysate inhibit melanogenesis in B16F0 cells by regulating the CREB/MITF/tyrosinase pathway.
MA5-14146 was used in western blot to study the molecular mechanism by which an aqueous alcoholic Rhodiola rosea extract inhibits melanogenesis in a murine melanoma cell line
|Chiang HM,Chien YC,Wu CH,Kuo YH,Wu WC,Pan YY,Su YH,Wen KC||Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (65:129)||2014|
Inhibitory effects of resveratrol on melanin synthesis in ultraviolet B-induced pigmentation in Guinea pig skin.
MA5-14146 was used in western blot to study the mechanism by which resveratrol inhibits melanin sythesis during UVB-induced skin pigmentation
|Lee TH,Seo JO,Baek SH,Kim SY||Biomolecules & therapeutics (22:35)||2014|
A polymorphism in IRF4 affects human pigmentation through a tyrosinase-dependent MITF/TFAP2A pathway.
MA5-14146 was used in western blot to study the molecular mechanism by which an IRF4 polymorphism affects pigmentation in humans
|Praetorius C,Grill C,Stacey SN,Metcalf AM,Gorkin DU,Robinson KC,Van Otterloo E,Kim RS,Bergsteinsdottir K,Ogmundsdottir MH,Magnusdottir E,Mishra PJ,Davis SR,Guo T,Zaidi MR,Helgason AS,Sigurdsson MI,Meltzer PS,Merlino G,Petit V,Larue L,Loftus SK,Adams DR,Sobhiafshar U,Emre NC,Pavan WJ,Cornell R,Smith AG,McCallion AS,Fisher DE,Stefansson K,Sturm RA,Steingrimsson E||Cell (155:1022)||2013|
Effect of SMURF2 targeting on susceptibility to MEK inhibitors in melanoma.
MA5-14146 was used in western blot to study the ability of SMURF2 knockdown the enhance the sensitivity of melanoma cells to MEK inhibitors
|Smith MP,Ferguson J,Arozarena I,Hayward R,Marais R,Chapman A,Hurlstone A,Wellbrock C||Journal of the National Cancer Institute (105:33)||2013|
Expression of microphthalmia-associated transcription factor (MITF), which is critical for melanoma progression, is inhibited by both transcription factor GLI2 and transforming growth factor-β.
MA5-14146 was used in western blot to study the mechanisms by which TGF-beta and the GLI2 transcription factor inhibit MITF expression and the significance for the progression of melanoma
|Pierrat MJ,Marsaud V,Mauviel A,Javelaud D||The Journal of biological chemistry (287:17996)||2012|
Inhibition of melanogenesis by Xanthium strumarium L.
MA5-14146 was used in western blot to study the mechanism by which an extract of Xanthium strumarium L inhibits the synthesis of melanin
|Li H,Min YS,Park KC,Kim DS||Bioscience, biotechnology, and biochemistry (76:767)||2012|
Anti-melanogenic property of geoditin A in murine B16 melanoma cells.
MA5-14146 was used in western blot to investigate the anti-melanogenic properties of geoditin A.
|Cheung FW,Guo J,Ling YH,Che CT,Liu WK||Marine drugs (10:465)||2012|
Identification of a melanocyte-specific, microphthalmia-associated transcription factor-dependent regulatory element in the intronic duplication causing hair greying and melanoma in horses.
MA5-14146 was used in western blot to study the role of a microphthalmia-associated transcription factor-dependent regulatory element in the intronic duplication causing hair greying and melanoma in horses
|Sundström E,Komisarczuk AZ,Jiang L,Golovko A,Navratilova P,Rinkwitz S,Becker TS,Andersson L||Pigment cell & melanoma research (25:28)||2012|
Essential role of microphthalmia transcription factor for DNA replication, mitosis and genomic stability in melanoma.
MA5-14146 was used in western blot to study the role of the microphthalmia transcription factor in melanoma pathogenesis
|Strub T,Giuliano S,Ye T,Bonet C,Keime C,Kobi D,Le Gras S,Cormont M,Ballotti R,Bertolotto C,Davidson I||Oncogene (30:2319)||2011|
WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition.
MA5-14146 was used in western blot to investigate the involvement of WIPI1 in transcriptional regulation of melanogenic gene and melanosome formation
|Ho H,Kapadia R,Al-Tahan S,Ahmad S,Ganesan AK||The Journal of biological chemistry (286:12509)||2011|
A single UVB exposure increases the expression of functional KIT in human melanocytes by up-regulating MITF expression through the phosphorylation of p38/CREB.
MA5-14146 was used in western blot to study the mechanism by which UVB exposure increases the expression of functional KIT in human melanocytes
|Mizutani Y,Hayashi N,Kawashima M,Imokawa G||Archives of dermatological research (302:283)||2010|
PAX3 and SOX10 activate MET receptor expression in melanoma.
MA5-14146 was used in western blot to investigate the role of SOX3,SOX10,MITF and MET in human melanoma tumorigenesis
|Mascarenhas JB,Littlejohn EL,Wolsky RJ,Young KP,Nelson M,Salgia R,Lang D||Pigment cell & melanoma research (23:225)||2010|
Effects of vitamin C vs. multivitamin on melanogenesis: comparative study in vitro and in vivo.
MA5-14146 was used in western blot to study the effects of vitamin C and multivitamin treatment on melanogenesis and hyperpigmentation
|Choi YK,Rho YK,Yoo KH,Lim YY,Li K,Kim BJ,Seo SJ,Kim MN,Hong CK,Kim DS||International journal of dermatology (49:218)||2010|
Frequent mutations in the MITF pathway in melanoma.
MA5-14146 was used in western blot to study frequently occuring mutations of MITF and SOX10 in melanoma
|Cronin JC,Wunderlich J,Loftus SK,Prickett TD,Wei X,Ridd K,Vemula S,Burrell AS,Agrawal NS,Lin JC,Banister CE,Buckhaults P,Rosenberg SA,Bastian BC,Pavan WJ,Samuels Y||Pigment cell & melanoma research (22:435)||2009|
Analysis of cultured human melanocytes based on polymorphisms within the SLC45A2/MATP, SLC24A5/NCKX5, and OCA2/P loci.
MA5-14146 was used in western blot to investigate the phenotypic correlation with single nucleotide polymorphisms within SLC45A2/MATP, SLC24A5/NCKX5 and OCA2/P loci
|Cook AL,Chen W,Thurber AE,Smit DJ,Smith AG,Bladen TG,Brown DL,Duffy DL,Pastorino L,Bianchi-Scarra G,Leonard JH,Stow JL,Sturm RA||The Journal of investigative dermatology (129:392)||2009|
Methyl-beta-cyclodextrin, a specific cholesterol-binding agent, inhibits melanogenesis in human melanocytes through activation of ERK.
MA5-14146 was used in western blot to examine the effect of cholesterol-binding agent methyl-beta-cyclodextrin in melanogenesis in human melanocytes
|Jin SH,Lee YY,Kang HY||Archives of dermatological research (300:451)||2008|
In vivo switching of human melanoma cells between proliferative and invasive states.
MA5-14146 was used in western blot to investigate the transition of human melanoma cells from a proliferative state to an invasive state
|Hoek KS,Eichhoff OM,Schlegel NC,Döbbeling U,Kobert N,Schaerer L,Hemmi S,Dummer R||Cancer research (68:650)||2008|
Osteonectin downregulates E-cadherin, induces osteopontin and focal adhesion kinase activity stimulating an invasive melanoma phenotype.
MA5-14146 was used in western blot to investigate the consequences of osteonectin expression in melanoma cells
|Smit DJ,Gardiner BB,Sturm RA||International journal of cancer (121:2653)||2007|
Role of microphthalmia transcription factor (Mitf) in melanoma differentiation.
MA5-14146 was used in western blot to investigate the involvement of microphthalmia transcription factor in melanoma differentiation
|Lekmine F,Chang CK,Sethakorn N,Das Gupta TK,Salti GI||Biochemical and biophysical research communications (354:830)||2007|
Involvement of ITF2 in the transcriptional regulation of melanogenic genes.
MA5-14146 was used in western blot to study the role of ubiquitous basic helix-loop-helix transcription factor ITF2 in melanogenic process
|Furumura M,Potterf SB,Toyofuku K,Matsunaga J,Muller J,Hearing VJ||The Journal of biological chemistry (276:28147)||2001|
Insertion of long interspersed element-1 in the Mitf gene is associated with altered neurobehavior of the black-eyed white Mitf(mi-bw) mouse.
MA5-14146 was used in immunohistochemistry to study the altered neurological behaviour of black-eyed white mice in response to environmental stress and the role of the long interspersed element-1 insertion in the Mitf gene
|Takeda K,Hozumi H,Nakai K,Yoshizawa M,Satoh H,Yamamoto H,Shibahara S||Genes to cells : devoted to molecular & cellular mechanisms (19:126)||2014|
Primary clear cell sarcoma of the tongue.
MA5-14146 was used in immunohistochemistry to report on a case of primary clear cell sarcoma of the tongue in an 82 year-old woman
|Kraft S,Antonescu CR,Rosenberg AE,Deschler DG,Nielsen GP||Archives of pathology & laboratory medicine (137:1680)||2013|
Defective FGF signaling causes coloboma formation and disrupts retinal neurogenesis.
MA5-14146 was used in immunohistochemistry to study the effects of conditional knockout of Fgfr1 and Fgfr2 on optic fissure closure and retinal neurogenesis in mice
|Chen S,Li H,Gaudenz K,Paulson A,Guo F,Trimble R,Peak A,Seidel C,Deng C,Furuta Y,Xie T||Cell research (23:254)||2013|
Cadherin-mediated cell adhesion is critical for the closing of the mouse optic fissure.
MA5-14146 was used in immunohistochemistry to study murine optic fissure closure and the role of cell adhesion mediated by cadherin
|Chen S,Lewis B,Moran A,Xie T||PloS one (7:null)||2012|
Impaired development of melanoblasts in the black-eyed white Mitf(mi-bw) mouse, a model for auditory-pigmentary disorders.
MA5-14146 was used in immunohistochemistry to study the defective development of melanoblasts observed in the black-eyed white Mitf(mi-bw) mouse
|Hozumi H,Takeda K,Yoshida-Amano Y,Takemoto Y,Kusumi R,Fukuzaki-Dohi U,Higashitani A,Yamamoto H,Shibahara S||Genes to cells : devoted to molecular & cellular mechanisms (17:494)||2012|
Intrinsic differences among spatially distinct neural crest stem cells in terms of migratory properties, fate determination, and ability to colonize the enteric nervous system.
MA5-14146 was used in immunohistochemistry to study the intrinsic differences among spatially distinct neural crest stem cells
|Mosher JT,Yeager KJ,Kruger GM,Joseph NM,Hutchin ME,Dlugosz AA,Morrison SJ||Developmental biology (303:1)||2007|
Single UVB overexposure stimulates melanocyte proliferation in murine skin, in contrast to fractionated or UVA-1 exposure.
MA5-14146 was used in immunohistochemistry to study the ability of a single UVB overexposure to stimulate melanocyte proliferation in murine skin
|van Schanke A,Jongsma MJ,Bisschop R,van Venrooij GM,Rebel H,de Gruijl FR||The Journal of investigative dermatology (124:241)||2005|
Paraganglioma-like dermal melanocytic tumor: a unique entity distinct from cellular blue nevus, clear cell sarcoma, and cutaneous melanoma.
MA5-14146 was used in immunohistochemistry to report on a study of paraganglioma-like dermal melanocytic tumor in 8 patients
|Deyrup AT,Althof P,Zhou M,Morgan M,Solomon AR,Bridge JA,Weiss SW||The American journal of surgical pathology (28:1579)||2004|
Immunoprofile of MITF, tyrosinase, melan-A, and MAGE-1 in HMB45-negative melanomas.
MA5-14146 was used in immunohistochemistry to investigate the expression of protein markers associated with melanoma and melanocytic differentiation in HMB45-negative melanomas
|Xu X,Chu AY,Pasha TL,Elder DE,Zhang PJ||The American journal of surgical pathology (26:82)||2002|
Microphthalmia transcription factor in the immunohistochemical diagnosis of metastatic melanoma: comparison with four other melanoma markers.
MA5-14146 was used in immunohistochemistry to study microphthalmia transcription factor as a diagnostic marker of metastatic melanoma.
|Miettinen M,Fernandez M,Franssila K,Gatalica Z,Lasota J,Sarlomo-Rikala M||The American journal of surgical pathology (25:205)||2001|
|Human||1 ug/million cells||
A simple and scalable process for the differentiation of retinal pigment epithelium from human pluripotent stem cells.
MA5-14146 was used in flow cytometry and immunocytochemistry to develop a protocol with potential for the large scale differentiation of human pluripotent stem cells into retinal pigment epithelial cells
|Maruotti J,Wahlin K,Gorrell D,Bhutto I,Lutty G,Zack DJ||Stem cells translational medicine (2:341)||2013|
The culture and maintenance of functional retinal pigment epithelial monolayers from adult human eye.
MA5-14146 was used in immunocytochemistry to develop a protocol for the isolation and expansion of monolayers of functional human retinal pigment epithelia obtained from adult eyes
|Blenkinsop TA,Salero E,Stern JH,Temple S||Methods in molecular biology (Clifton, N.J.) (945:45)||2012|
Elevated expression of MITF counteracts B-RAF-stimulated melanocyte and melanoma cell proliferation.
MA5-14146 was used in immunocytochemistry and western blot to study the role of MITF in counteracting B-RAF-stimulated melanocyte and melanoma cell proliferation
|Wellbrock C,Marais R||The Journal of cell biology (170:703)||2005|
Activation of p59(Fyn) leads to melanocyte dedifferentiation by influencing MKP-1-regulated mitogen-activated protein kinase signaling.
MA5-14146 was used in immunocytochemistry to study the mechanism by which the activation of p59Fyn leads to melanocyte de-differentiation
|Wellbrock C,Weisser C,Geissinger E,Troppmair J,Schartl M||The Journal of biological chemistry (277:6443)||2002|
SOX9, through interaction with microphthalmia-associated transcription factor (MITF) and OTX2, regulates BEST1 expression in the retinal pigment epithelium.
MA5-14146 was used in immunoprecipitation and western blot to study the mechanism by which SOX9 regulates BEST1 expression in the retinal pigment epithelium
|Masuda T,Esumi N||The Journal of biological chemistry (285:26933)||2010|
VMD2 promoter requires two proximal E-box sites for its activity in vivo and is regulated by the MITF-TFE family.
MA5-14146 was used in EMSA to study the role of E-box sites and microphthalmia-associated transcription factors in the in vivo activity of the VMD2 promoter
|Esumi N,Kachi S,Campochiaro PA,Zack DJ||The Journal of biological chemistry (282:1838)||2007|
black eyed white; class E basic helix-loop-helix protein 32; microphtalmia-associated transcription factor; microphthalmia transcription factor; microphthalmia-associated transcription factor; microphthalmia-associated transcription factor variant MITF-M(+)
BCC2; BHLHE32; Bw; CMM8; Gsfbcc2; MI; MITF; MITF-H; MITF-M; Vit; Vitiligo; Wh; WS2; WS2A