Flow cytometry analysis of Microglobulin Beta-2 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Microglobulin Beta-2 monoclonal antibody (Product # MA5-13418) at a dilution of 1ug/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human PBL (T-ALL patient)|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1ug/test|
|Immunofluorescence (IF)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13418 targets Microglobulin Beta-2 in FACS and IF applications and shows reactivity with Human samples.
The MA5-13418 immunogen is human PBL (T-ALL patient).
beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. Clone HLA-ABC-m2 (also known as 246-E8.E7) is specific to human beta-microglobulin and does not react with primate cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Endothelial cells from cord blood CD133+CD34+ progenitors share phenotypic, functional and gene expression profile similarities with lymphatics.
MA5-13418 was used in flow cytometry to study the phenotype, function and gene expression profile of endothelial cells from cord blood CD133+CD34+ progenitors
|Nguyen VA,Fürhapter C,Obexer P,Stössel H,Romani N,Sepp N||Journal of cellular and molecular medicine (13:522)||2009|
Loss of heterozygosity in the HLA class I region in human pancreatic cancer.
MA5-13418 was used in immunohistochemistry to study loss of heterozygosity in the HLA class I region in 24 patients with ductal pancreatic carcinoma
|Ryschich E,Cebotari O,Fabian OV,Autschbach F,Kleeff J,Friess H,Bierhaus A,B?chler MW,Schmidt J||Tissue antigens (64:696)||2004|