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|Tested species reactivity||Dog, Human|
|Published species reactivity||Sheep, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||N-terminal fragment of human Mi protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Gel Shift (GS)||1mg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||4-8 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14154 targets Microphthalmia-Transcription Factor in GS and IHC (P) applications and shows reactivity with Canine and Human samples.
The MA5-14154 immunogen is n-terminal fragment of human Mi protein.
Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Unusual clear cell, lymphoplasmacyte-rich, dural-based tumor with divergent differentiation: a tricky case mimicking a meningioma.
MA5-14154 was used in immunohistochemistry - paraffin section to describe an unusual case of a recurrent dural neoplasm
|Miracco C,Toscano M,Butorano MA,Baldino G,Tacchini D,Barone A,Cerase A||Human pathology (46:1050)||2015|
PRMT5 is upregulated in malignant and metastatic melanoma and regulates expression of MITF and p27(Kip1.).
MA5-14154 was used in western blot to study the increased immunohistochemical expression of PRMT5 in malignant and metastatic melanoma and the pathological significance
|Nicholas C,Yang J,Peters SB,Bill MA,Baiocchi RA,Yan F,Sïf S,Tae S,Gaudio E,Wu X,Grever MR,Young GS,Lesinski GB||PloS one (8:null)||2013|
Secondary c-Kit mutations confer acquired resistance to RTK inhibitors in c-Kit mutant melanoma cells.
MA5-14154 was used in western blot to study the acquisition of resistance to RTK inhibitors by c-Kit mutant melanoma cells through secondary c-Kit mutations and the therapeutic implications
|Todd JR,Becker TM,Kefford RF,Rizos H||Pigment cell & melanoma research (26:518)||2013|
Melanoma cell invasiveness is regulated by miR-211 suppression of the BRN2 transcription factor.
MA5-14154 was used in western blot to investigate the mechanism for the regulation of melanoma invasiveness
|Boyle GM,Woods SL,Bonazzi VF,Stark MS,Hacker E,Aoude LG,Dutton-Regester K,Cook AL,Sturm RA,Hayward NK||Pigment cell & melanoma research (24:525)||2011|
MicroRNA-25 functions in regulation of pigmentation by targeting the transcription factor MITF in Alpaca (Lama pacos) skin melanocytes.
MA5-14154 was used in western blot to study the mechanism by which microRNA-25 regulates pigmentation in Alpaca skin melanocytes
|Zhu Z,He J,Jia X,Jiang J,Bai R,Yu X,Lv L,Fan R,He X,Geng J,You R,Dong Y,Qiao D,Lee KB,Smith GW,Dong C||Domestic animal endocrinology (38:200)||2010|
Macrophage inhibitory cytokine-1 is overexpressed in malignant melanoma and is associated with tumorigenicity.
MA5-14154 was used in western blot to study the role of macrophage inhibitory cytokine-1 overexpression in malignant melanoma
|Boyle GM,Pedley J,Martyn AC,Banducci KJ,Strutton GM,Brown DA,Breit SN,Parsons PG||The Journal of investigative dermatology (129:383)||2009|
Melanocytes expressing MC1R polymorphisms associated with red hair color have altered MSH-ligand activated pigmentary responses in coculture with keratinocytes.
MA5-14154 was used in western blot to study the behavior and functional abilities of melanocytes expressing MC1R red hair color variants
|Roberts DW,Newton RA,Leonard JH,Sturm RA||Journal of cellular physiology (215:344)||2008|
MicroRNA-137 targets microphthalmia-associated transcription factor in melanoma cell lines.
MA5-14154 was used in western blot to confirm the role of miR-137 in the regulation of microphthalmia-associated transcription factor expression
|Bemis LT,Chen R,Amato CM,Classen EH,Robinson SE,Coffey DG,Erickson PF,Shellman YG,Robinson WA||Cancer research (68:1362)||2008|
Melanocytic differentiation is present in a significant proportion of nonpigmented diffuse neurofibromas: a potential diagnostic pitfall.
MA5-14154 was used in immunohistochemistry to study the occurrence of melanocytic differentiation in non-pigmented diffuse neurofibromas and the diagnostic implications
|Pižem J,Nicholson KM,Mraz J,Prieto VG||The American journal of surgical pathology (37:1182)||2013|
Cathepsin K expression in the spectrum of perivascular epithelioid cell (PEC) lesions of the kidney.
MA5-14154 was used in immunohistochemistry to investigate the expression of cathepsin K in kidney perivascular epithelioid cell lesions
|Martignoni G,Bonetti F,Chilosi M,Brunelli M,Segala D,Amin MB,Argani P,Eble JN,Gobbo S,Pea M||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (25:100)||2012|
MART-1 is a reliable melanocytic marker in lichen planus-like keratosis: a study on 70 cases.
MA5-14154 was used in immunohistochemistry to evaluate the suitability of MART-1 as a marker for melanocytic nests in lichen planus-like keratosis
|Gavino AC,Woods MT,Andea AA||The American Journal of dermatopathology (33:675)||2011|
PAX3 and SOX10 activate MET receptor expression in melanoma.
MA5-14154 was used in immunohistochemistry to investigate the role of SOX3,SOX10,MITF and MET in human melanoma tumorigenesis
|Mascarenhas JB,Littlejohn EL,Wolsky RJ,Young KP,Nelson M,Salgia R,Lang D||Pigment cell & melanoma research (23:225)||2010|
Low-grade sarcoma in classical seminoma - the first case reported.
MA5-14154 was used in immunohistochemistry to report on a case of low-grade sarcoma in classical seminoma
|Petersson F,Michal M,Grossmann P,Franco M,Zámecník M,Hes O||International journal of clinical and experimental pathology (3:203)||2010|
Cathepsin-K immunoreactivity distinguishes MiTF/TFE family renal translocation carcinomas from other renal carcinomas.
MA5-14154 was used in immunohistochemistry to examine the diagnostic significance of cathepsin-K immunoreactivity in MiTF/TFE family renal translocation carcinomas
|Martignoni G,Pea M,Gobbo S,Brunelli M,Bonetti F,Segala D,Pan CC,Netto G,Doglioni C,Hes O,Argani P,Chilosi M||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (22:1016)||2009|
Metastatic desmoplastic malignant melanoma associated with low-grade myofibroblastic sarcoma.
MA5-14154 was used in immunohistochemistry to report on a case of metastatic desmoplastic malignant melanoma associated with low-grade myofibroblastic sarcoma
|Kacerovska D,Michal M,Kutzner H,Rychnovsky J,Kazakov DV||The American Journal of dermatopathology (31:490)||2009|
Cathepsin-k expression in pulmonary lymphangioleiomyomatosis.
MA5-14154 was used in immunohistochemistry to examine the role of cathepsin-k in the pathogenesis of pulmonary lymphangioleiomyomatosis
|Chilosi M,Pea M,Martignoni G,Brunelli M,Gobbo S,Poletti V,Bonetti F||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (22:161)||2009|
Clear cell sarcoma of soft tissue: a clinicopathologic, immunohistochemical, and molecular analysis of 33 cases.
MA5-14154 was used in immunohistochemistry to analyse 33 clinical cases of clear cell sarcoma of soft tissue
|Hisaoka M,Ishida T,Kuo TT,Matsuyama A,Imamura T,Nishida K,Kuroda H,Inayama Y,Oshiro H,Kobayashi H,Nakajima T,Fukuda T,Ae K,Hashimoto H||The American journal of surgical pathology (32:452)||2008|
Biphasic malignant mesothelioma cases with osseous differentiation and long survival: a review of the literature.
MA5-14154 was used in immunohistochemistry to report two clinical cases of biphasic malignant mesothelioma with osseous differentiation
|Demirag F,Unsal E,Tastepe I||Lung cancer (Amsterdam, Netherlands) (57:233)||2007|
Distinct phenotypic changes between the superficial and deep component of giant congenital melanocytic naevi: a rationale for curettage.
MA5-14154 was used in immunohistochemistry to study the phenotypic changes between the superficial and deep component of giant congenital melanocytic naevi
|De Raeve LE,Claes A,Ruiter DJ,van Muijen GN,Roseeuw D,van Kempen LC||The British journal of dermatology (154:485)||2006|
Mechanisms of skin tanning in different racial/ethnic groups in response to ultraviolet radiation.
MA5-14154 was used in immunohistochemistry to study the mechanisms by which UV radiation stimulates skin pigmentation in different ethnic groups
|Tadokoro T,Yamaguchi Y,Batzer J,Coelho SG,Zmudzka BZ,Miller SA,Wolber R,Beer JZ,Hearing VJ||The Journal of investigative dermatology (124:1326)||2005|
Immunohistochemical analysis of novel monoclonal antibody PNL2 and comparison with other melanocyte differentiation markers.
MA5-14154 was used in immunohistochemistry to investigate the usefulness of a novel antibody PNL2 for melanocyte staining
|Busam KJ,Kucukgöl D,Sato E,Frosina D,Teruya-Feldstein J,Jungbluth AA||The American journal of surgical pathology (29:400)||2005|
Paraganglioma-like dermal melanocytic tumor: a unique entity distinct from cellular blue nevus, clear cell sarcoma, and cutaneous melanoma.
MA5-14154 was used in immunohistochemistry to report on a study of paraganglioma-like dermal melanocytic tumor in 8 patients
|Deyrup AT,Althof P,Zhou M,Morgan M,Solomon AR,Bridge JA,Weiss SW||The American journal of surgical pathology (28:1579)||2004|
Congenital giant melanocytic nevus with pigmented epithelioid cells: a variant of epithelioid blue nevus.
MA5-14154 was used in immunohistochemistry to report a case study of a boy with a giant melanocytic variant of epithelioid blue nevus.
|Martínez-Barba E,Polo-García LA,Ferri-Níguez B,Ruiz-Maciá JA,Kutzner H,Requena L||The American Journal of dermatopathology (24:30)||2002|
Immunoprofile of MITF, tyrosinase, melan-A, and MAGE-1 in HMB45-negative melanomas.
MA5-14154 was used in immunohistochemistry to investigate the expression of protein markers associated with melanoma and melanocytic differentiation in HMB45-negative melanomas
|Xu X,Chu AY,Pasha TL,Elder DE,Zhang PJ||The American journal of surgical pathology (26:82)||2002|
Expression of melanocytic differentiation markers in malignant melanomas of the oral and sinonasal mucosa.
MA5-14154 was used in immunohistochemistry to study the expression of melanocytic differentiation markers in oral and sinonasal melanomas
|Prasad ML,Jungbluth AA,Iversen K,Huvos AG,Busam KJ||The American journal of surgical pathology (25:782)||2001|
Microphthalmia transcription factor in the immunohistochemical diagnosis of metastatic melanoma: comparison with four other melanoma markers.
MA5-14154 was used in immunohistochemistry to study microphthalmia transcription factor as a diagnostic marker of metastatic melanoma.
|Miettinen M,Fernandez M,Franssila K,Gatalica Z,Lasota J,Sarlomo-Rikala M||The American journal of surgical pathology (25:205)||2001|
VMD2 promoter requires two proximal E-box sites for its activity in vivo and is regulated by the MITF-TFE family.
MA5-14154 was used in EMSA to study the role of E-box sites and microphthalmia-associated transcription factors in the in vivo activity of the VMD2 promoter
|Esumi N,Kachi S,Campochiaro PA,Zack DJ||The Journal of biological chemistry (282:1838)||2007|
class E basic helix-loop-helix protein 32; microphtalmia-associated transcription factor
BHLHE32; CMM8; MI; MITF; MITF-H; MITF-M; WS2; WS2A