|Gel Shift (GS)||1mg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||4-8 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Western Blot (WB)||See 7 publications below|
|Immunohistochemistry (IHC)||See 19 publications below|
|Gel Shift (GS)||See 1 publications below|
|Tested Species reactivity||Dog, Human|
|Published species reactivity||Sheep Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||N-terminal fragment of human Mi protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-14154 targets Microphthalmia-Transcription Factor in GS and IHC (P) applications and shows reactivity with Canine and Human samples.
The MA5-14154 immunogen is n-terminal fragment of human Mi protein.
Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bHLHe32; Class E basic helix-loop-helix protein 32; microphtalmia-associated transcription factor; Microphthalmia-associated transcription factor
Gene Aliases: BHLHE32; CMM8; MI; MITF; MITF-H; MITF-M; WS2; WS2A
UniProt ID: (Human) O75030
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