|Tested species reactivity||Mouse|
|Host / Isotype||Mouse / IgG|
|Immunogen||Mouse Mist1, N-terminus residues 1-20.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-518 detects Mist1 from mouse samples. MA1-518 has been successfully used in Western blot. By Western blot, MA1-518 detects a ~29 kDa band representing Mist1. The MA1-518 immunogen is mouse Mist1, N-terminus residues 1-20.
Mist 1 plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. This protein also negatively regulates bHLH-mediated transcription through an N-terminal repressor domain, and serves as a key regulator of acinar cell function, stability, and identity.
Mist 1 is also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport, and may function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.