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Immunofluorescent analysis of Mitosin in U2OS cells fixed by 4% PFA. Cells were costained using a Mitosin monoclonal antibody (Product # MA1-23185) at a dilution of 1:500 and an Alpha-Tubulin polyclonal antibody (Product # PA5-29444) (Green) at a dilution of 1:500. Chromosomes are stained with DAPI (blue). Scale bar, 5 um.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23185 detects mitosin in Human samples.
MA1-23185 has been successfully used in Immunofluorescence, Immunohistochemistry (frozen and paraffin), Immunoprecipitation and Western Blot procedures.
MA1-23185 immunogen corresponds to Protein fragment expressed in E. coli corresponding to amino acids 1759-2093..
Mitosin-14C10 recognizes mitosin, a 350kDa phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase, and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AH antigen; cell-cycle-dependent 350K nuclear protein; CENF; CENP-F; CENP-F kinetochore protein; Centromere protein F; centromere protein F, 350/400ka (mitosin); centromere protein F, 350/400kDa (mitosin); hcp-1; Kinetochore protein CENPF; Mitosin; PRO1779; RP11-262H5.1
CENF; CENPF; CILD31; hcp-1; PRO1779; STROMS