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|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Total cell lysate of the human bladder carcinoma cell line T24.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-06200 detects Mitotic cells in human samples.
MA1-06200 has sucessfully been used in immunocytochemistry and FACS procedures.
The MA1-06200 immunogen is total cell lysate of the human bladder carcinoma cell line T24.
The life cycle of a eukaryotic cell consists of various phases, two of which can morphologically and biochemically be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques, such as the BrdU incorporation assay. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a "e;quiescent"e; phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S, and from G2 to M-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.