|Tested species reactivity||Bovine, Human, Rat|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Moesin purified from cow uterus|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||0.25-0.5 µg/mL|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14040 targets Moesin in IF, IHC (P), IP, and WB applications and shows reactivity with Bovine, Human, and Rat samples.
The MA5-14040 immunogen is moesin purified from cow uterus.
Moesin, a member of the talin-4.1 superfamily, is a linking protein of the submembraneous actin cytoskeleton. It is expressed in macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. It is involved in cell adhesion, migration, and organization of cell surface structures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Signal-dependent slow leukocyte rolling does not require cytoskeletal anchorage of P-selectin glycoprotein ligand-1 (PSGL-1) or integrin αLβ2.
MA5-14040 was used in western blot to study whether cytoskelatal-anchorage of PSGL-1 and alphaLbeta2 intergrin is required for signal-dependent slow leukocyte rolling
|Shao B,Yago T,Coghill PA,Klopocki AG,Mehta-D'souza P,Schmidtke DW,Rodgers W,McEver RP||The Journal of biological chemistry (287:19585)||2012|
The effect of sphingomyelin synthase 2 (SMS2) deficiency on the expression of drug transporters in mouse brain.
MA5-14040 was used in western blot to identify the relationship between sphingomyelin synthetase 2 levels and drug transporter expression
|Zhang Y,Dong J,Zhu X,Wang W,Yang Q||Biochemical pharmacology (82:287)||2011|
Distinct cellular and therapeutic effects of obatoclax in rituximab-sensitive and -resistant lymphomas.
MA5-14040 was used in western blot to study the cellular and therapeutic effects of obatoclax in rituximab-sensitive and -resistant lymphomas
|Brem EA,Thudium K,Khubchandani S,Tsai PC,Olejniczak SH,Bhat S,Riaz W,Gu J,Iqbal A,Campagna R,Knight J,Mavis C,Hoskin P,Deeb G,Gibbs JF,Fetterly G,Czuczman MS,Hernandez-Ilizaliturri FJ||British journal of haematology (153:599)||2011|
Distinct molecular mechanisms responsible for bortezomib-induced death of therapy-resistant versus -sensitive B-NHL cells.
MA5-14040 was used in western blot to study the molecular mechanisms responsible for bortezomib-induced death of therapy-resistant versus -sensitive B-NHL cells
|Olejniczak SH,Blickwedehl J,Belicha-Villanueva A,Bangia N,Riaz W,Mavis C,Clements JL,Gibbs J,Hernandez-Ilizaliturri FJ,Czuczman MS||Blood (116:5605)||2010|
TREM-like transcript-1 protects against inflammation-associated hemorrhage by facilitating platelet aggregation in mice and humans.
MA5-14040 was used in western blot to investigate the protective effect of TREM-like transcript-1 -1 against inflammation-associated hemorrhage
|Washington AV,Gibot S,Acevedo I,Gattis J,Quigley L,Feltz R,De La Mota A,Schubert RL,Gomez-Rodriguez J,Cheng J,Dutra A,Pak E,Chertov O,Rivera L,Morales J,Lubkowski J,Hunter R,Schwartzberg PL,McVicar DW||The Journal of clinical investigation (119:1489)||2009|
Acquired resistance to rituximab is associated with chemotherapy resistance resulting from decreased Bax and Bak expression.
MA5-14040 was used in western blot to study the role of Bax and Bak downregulation in the drug resistance to rituximab
|Olejniczak SH,Hernandez-Ilizaliturri FJ,Clements JL,Czuczman MS||Clinical cancer research : an official journal of the American Association for Cancer Research (14:1550)||2008|
Specific antibodies to moesin, a membrane-cytoskeleton linker protein, are frequently detected in patients with acquired aplastic anemia.
MA5-14040 was used in western blot to identify novel auto-antibodies in acquired aplastic anemia
|Takamatsu H,Feng X,Chuhjo T,Lu X,Sugimori C,Okawa K,Yamamoto M,Iseki S,Nakao S||Blood (109:2514)||2007|
Anti-moesin antibodies in the serum of patients with aplastic anemia stimulate peripheral blood mononuclear cells to secrete TNF-alpha and IFN-gamma.
MA5-14040 was used in flow cytometry to study the role of anti-moesin antibodies in the secretion of TNF-alpha and IFN-gamma by PBMCs in patients with aplastic anemia
|Takamatsu H,Espinoza JL,Lu X,Qi Z,Okawa K,Nakao S||Journal of immunology (Baltimore, Md. : 1950) (182:703)||2009|
Age-related defects in moesin/ezrin cytoskeletal signals in mouse CD4 T cells.
MA5-14040 was used in flow cytometry and western blot to study age-related defects in moesin/Ezrin cytoskeletal signals in mouse CD4 T cells
|Garcia GG,Sadighi Akha AA,Miller RA||Journal of immunology (Baltimore, Md. : 1950) (179:6403)||2007|
Adhesion molecules as prognostic markers in pancreatic adenocarcinoma.
MA5-14040 was used in immunohistochemistry to study the prognostic value of ezrin, radixin, moesin and E-cadherin in pancreatic ductal adenocarcinoma
|Torer N,Kayaselcuk F,Nursal TZ,Yildirim S,Tarim A,Nòyan T,Karakayali H||Journal of surgical oncology (96:419)||2007|
Differential tissue and subcellular expressionof ERM proteins in normal and malignant tissues: cytoplasmic ezrin expression has prognostic signficance for head and neck squamous cell carcinoma.
MA5-14040 was used in immunohistochemistry to examine the prognostic significance of cytoplasmic ezrin expression for head and neck squamous cell carcinoma
|Madan R,Brandwein-Gensler M,Schlecht NF,Elias K,Gorbovitsky E,Belbin TJ,Mahmood R,Breining D,Qian H,Childs G,Locker J,Smith R,Haigentz M,Gunn-Moore F,Prystowsky MB||Head and neck (28:1018)||2006|
Ezrin promotes ovarian carcinoma cell invasion and its retained expression predicts poor prognosis in ovarian carcinoma.
MA5-14040 was used in immunohistochemistry to study the role of ezrin in ovarian carcinoma cell invasion
|Köbel M,Gradhand E,Zeng K,Schmitt WD,Kriese K,Lantzsch T,Wolters M,Dittmer J,Strauss HG,Thomssen C,Hauptmann S||International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (25:121)||2006|
Ezrin is essential for epithelial organization and villus morphogenesis in the developing intestine.
MA5-14040 was used in immunohistochemistry to study the essential role of Ezrin in epithelial organization and villus morphogenesis during intestinal development
|Saotome I,Curto M,McClatchey AI||Developmental cell (6:855)||2004|
Molecular profiling of bladder cancer using cDNA microarrays: defining histogenesis and biological phenotypes.
MA5-14040 was used in immunohistochemistry to study the molecular profile of bladder cancer using cDNA microarrays
|Sanchez-Carbayo M,Socci ND,Charytonowicz E,Lu M,Prystowsky M,Childs G,Cordon-Cardo C||Cancer research (62:6973)||2002|
Ezrin and moesin expression within the developing human cerebrum and tuberous sclerosis-associated cortical tubers.
MA5-14040 was used in immunohistochemistry to study the expression of ezrin and moesin in developing human cerebrum and tuberous sclerosis-associated cortical tubers
|Johnson MW,Miyata H,Vinters HV||Acta neuropathologica (104:188)||2002|
Chemokine stimulation of human peripheral blood T lymphocytes induces rapid dephosphorylation of ERM proteins, which facilitates loss of microvilli and polarization.
MA5-14040 was used in immunocytochemistry to investigate the regulation of chemokines on Carboxyphosphorylated-ERM and its effect on the morphology and activation of human peripheral blood T lymphocytes
|Brown MJ,Nijhara R,Hallam JA,Gignac M,Yamada KM,Erlandsen SL,Delon J,Kruhlak M,Shaw S||Blood (102:3890)||2003|