Invitrogen

Moesin Monoclonal Antibody (2C12)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (20) Moesin antibodies

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Datasheet

Protocols

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Moesin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded colon tissues using MSN monoclonal antibody (Product # MA5-17130) followed with DAB staining.

Moesin Antibody in Western Blot (WB) — Western blot analysis of Moesin was performed by loading 25 µg of WT (lane 1) and MSN CRISPR KO (lane 2) HeLa cell lysates in RIPA buffer onto a 4-15% gradient polyacrylamide gel. Proteins were transferred to nitrocellulose membrane and blocked in 5% milk. Ponceau stained transfer of blot is shown. Moesin was detected at approximately 68 kDa using a Moesin monoclonal antibody (Product # MA5-17130) at a dilution of 1:1,000 in 5% BSA in TBST overnight at 4 deg, followed by secondary antibody diluted to 0.2 µg/mL using Goat anti-Mouse IgG (H+L) HRP antibody (Product # 62-6520). Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Product # 32106). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.

Moesin Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Jurkat cells using MSN monoclonal antibody (Product # MA5-17130) (green) and negative control (red).

Moesin Antibody in Immunoprecipitation (IP) — Immunoprecipitation of Moesin was performed on HeLa cell lysates. Antibody-bead conjugates were prepared by adding 1 µg of Moesin monoclonal antibody (Product # MA5-17130) with 30 µL of protein G-Sepharose beads and rocked overnight at 4°C. 1 µg of MSN KO lysate was incubated with antibody-bead conjugate for 2 hrs at 4°C. After multiple washes, 10% starting material (SM), 10% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a Moesin monoclonal antibody. Ponceau stained transfer of blot is shown. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.

Moesin Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in MSN knockout (KO) cell line using Anti-Moesin monoclonal antibody (Product # MA5-17130). Ponceau stained transfer shown below. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation. {KO}

Product Details

MA5-17130

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:1,000	-
Flow Cytometry (Flow)	1:200 - 1:400	-
ELISA (ELISA)	1:10,000	-
Immunoprecipitation (IP)	1 µg antibody per 1 µg lysate	-

Product Specifications
Species Reactivity	Human, Non-human primate
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	2C12
Immunogen	Purified recombinant fragment of human MSN expressed in E. Coli.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2538601

Product Specific Information

MA5-17130 targets MSN in FACS, IHC, pep-ELISA, and WB applications and shows reactivity with Human and Non-human primate samples.

The MA5-17130 immunogen is purified recombinant fragment of human MSN expressed in E. Coli.

MA5-17130 detects MSN which has a predicted molecular weight of approximately 67.8kDa.

Target Information

Moesin, a member of the talin-4. 1 superfamily, is a linking protein of the submembraneous actin cytoskeleton. It is expressed in macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. It is involved in cell adhesion, migration, and organization of cell surface structures.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: epididymis luminal protein 70; Membrane-organizing extension spike protein; Moesin

Gene Aliases: HEL70; MSN

UniProt ID: (Human) P26038

Entrez Gene ID: (Human) 4478

Molecular Function: actin or actin-binding cytoskeletal protein

Function(s)

movement of cell or subcellular component cytoskeleton organization leukocyte cell-cell adhesion regulation of cell shape regulation of cell size positive regulation of gene expression gland morphogenesis membrane to membrane docking establishment of epithelial cell apical/basal polarity leukocyte migration establishment of endothelial barrier cellular response to testosterone stimulus positive regulation of podosome assembly regulation of organelle assembly positive regulation of protein localization to early endosome positive regulation of cellular protein catabolic process regulation of lymphocyte migration positive regulation of early endosome to late endosome transport

Process(es)

double-stranded RNA binding actin binding receptor binding structural constituent of cytoskeleton protein binding protein kinase binding cell adhesion molecule binding

Moesin Monoclonal Antibody (2C12)

Product Details

MA5-17130

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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