NeoBiotechnologies

Moesin Monoclonal Antibody (MSN, 491)

View all (20) Moesin antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

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Moesin Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis of PFA-fixed HeLa cells using Moesin Monoclonal Antibody (Product # 4478-MSM1-P1) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Moesin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human placenta using Moesin Monoclonal Antibody (Product # 4478-MSM1-P1).

Moesin Antibody in Western Blot (WB) — Western Blot analysis of human PC3 cell lysate using Moesin Monoclonal Antibody (Product # 4478-MSM1-P1).

Moesin Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of K562 cells using Moesin Monoclonal Antibody (Product # 4478-MSM1-P1) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Moesin Antibody — SDS-PAGE Analyis Purified Moesin Monoclonal Antibody (Product # 4478-MSM1-P1). Confirmation of Purity and Integrity of Antibody.

Product Details

4478-MSM1-P1

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1-2 µg/mL	-
Immunocytochemistry (ICC/IF)	2-4 µg/mL	-
Flow Cytometry (Flow)	1-2 µg/10^6 cells	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	MSN, 491
Immunogen	Recombinant full-length human Moesin protein View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	200 µg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	4° C

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

Moesin, a member of the talin-4. 1 superfamily, is a linking protein of the submembraneous actin cytoskeleton. It is expressed in macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. It is involved in cell adhesion, migration, and organization of cell surface structures.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.