Description: Mouse IL-1 beta, also called lymphocyte activating factor (LAF), endogenous pyrogen (EP), leucocyte endogenous mediator (LEM), mononuclear cell factor (MCF), is a ~17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1\beta is mostly cell associated with 23% amino acid homology with IL-1 alpha. The immune regulatory role of IL-1\beta is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.
Applications Reported: Recombinant mouse IL-1 beta has been reported for use in cytokine bioassays. This recombinant protein is biologically active.
Applications Tested: This reagent has been tested by sandwich ELISA using the anti-mouse IL-1 beta ELISA pair (B122/biotin-polyclonal) and in bioassays, with an observed ED50 of 10 pg/mL, corresponding to a specific activity of approximately 1x10e8 Units/mg.
Source: E. coli expressed amino acids Val 118-Ser 269 of mouse IL-1 beta accession # P10749.
Bioactivity: Measured by mouse D10S cell proliferation assay. The ED50 is 0.01 ng/mL, corresponding to a specific activity of 1.0 x 10e8 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Molecular Weight: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,393. The DTT reduced and non-reduced proteins migrate as 18 kDa polypeptides on SDS-PAGE.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IL-1 beta; Interleukin-1 beta
Gene Aliases: Il-1b; IL-1beta; Il1b
UniProt ID: (Mouse) P10749
Entrez Gene ID: (Mouse) 16176