Description: Mouse IL-10 is an ~18 kDa cytokine, also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.
Applications Reported: Recombinant mouse IL-10 is biologically active and has been verified to promote proliferation of the MC/9 cell line in culture.
Applications Tested: This recombinant mouse IL-10 has been tested by bioassay using the mouse cell line MC/9. The ED50 measured is typically 300 pg/mL, corresponding to a specific activity of 3.3x10e6 Units/mg.
Source: E. coli expressed amino acids Ser 19-Ser 178 of mature mouse IL-10 accession # NM_010548 with a Cys149tyr substitution.
Bioactivity: Measured by mouse MC/9 cell proliferation assay, costimulated with IL-4. The ED50 is 0.3 ng/mL, corresponding to a specific activity of 3.0 x 10e6 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Molecular Weight: The protein does not contain an N-terminal methionine. The polypeptide has a predicted molecular mass of approximately 18,950. The DTT reduced protein migrates as a 19 kDa polypeptide. The nonreduced protein migrates as an 18 kDa protein on non-reducing SDS-PAGE.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; IL-10; Interleukin-10
Gene Aliases: CSIF; Il-10; Il10
UniProt ID: (Mouse) P18893
Entrez Gene ID: (Mouse) 16153