Description: Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a ~21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.
Applications Reported: Recombinant mouse IL-6 is biologically active and can promote proliferation of mouse B cell lines in culture.
Applications Tested: The ED50 of this protein, as measured by B9 cell proliferation assay, is less than or equal to 5 pg/mL. This corresponds to a specific activity of greater than or equal to 2 x 10e8 Units/mg.
Source: E. coli expressed amino acids Phe 25-Thre 211 of mature mouse IL-6 accession # MMINTL6.
Bioactivity: The ED50 of this protein, as measured by B9 cell proliferation assay, is less than or equal to 5 pg/mL. This corresponds to a specific activity of greater than or equal to 2 x 10e8 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Molecular Weight: The protein is methionylated at the N-terminal. The DTT reduced and non-reduced protein migrate as 19 kDa polypeptides on SDS-PAGE.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-cell hybridoma growth factor; IL-6; Interleukin HP-1; Interleukin-6
Gene Aliases: Il-6; Il6
UniProt ID: (Mouse) P08505
Entrez Gene ID: (Mouse) 16193