Flow cytometry analysis of cytoplasmic immunoglobulin (Ig) lambda light chain expression was performed on Ramos (surface and cytoplasmic IgG/lambda-expressing B cells), Raji (cytoplasmic IgG/kappa-expressing B cells), and Jurkat (T cells) cell lines. Fixed and permeabilized cells were stained with a with a mouse anti-human Ig lambda light chain monoclonal antibody (Product # MA5-11590, blue histogram), with a mouse anti-human Ig kappa light chain monoclonal antibody (Product # MA5-12117, red histogram), each at a concentration of 1ug/ml. After incubation of the primary antibody on ice for 1 hour, the cells were stained with a FITC-conjugated goat anti-mouse IgG Fc secondary antibody (Product # 31630) at a dilution of 1:500 for 30 minutes on ice. The representative 10,000 cells were obtained for each sample.
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 15mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Cross Adsorption||Against human, bovine and rabbit serum proteins|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50 - 1:200|
|Immunocytochemistry (ICC)||1:50 - 1:200|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunohistochemistry (IHC)||1:50 - 1:200|
|Immunoprecipitation (IP)||1:50 - 1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31630 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31630 reacts with the heavy chains of mouse IgG subclasses 1, 2a, 2b, and 3, but not with light chains common to most mouse immunoglobulins. This antibody does not react against mouse IgM, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine, and rabbit serum proteins. However, these antibodies may cross-react with immunoglobulins from other species.
Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: 7.7 ug/mg; ~ 3 moles FITC per mole IgG (lot-dependent).
Reconstitute with 0.8 ml of distilled water (1.4 mg/ml after restoration).
Country of Origin: USA
Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.