Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1) and K-562 (Lane 2). The blots were probed with Anti SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 2 µg/ml) and detected by chemiluminescence using Donkey anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP (Product # SA1-100) at dilutions 1:1,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Donkey / IgG|
|Storage buffer||PBS with 50% glycerol, proprietary stabilizer|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Cross Adsorption||Against bovine, chicken, goat, guinea pig, syrian hamster, horse, human, rabbit and sheep serum proteins|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1,000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Product SA1-100 is conjugated to HRP and optimized for ECL applications. This antibody reacst with the heavy and light chains of the immunoglobulin molecule. Pre-absorbed with the following species proteins: Bovine, chicken, goat, guinea pig, syrian hamster, horse, human, rabbit, and sheep serum proteins.
Thermo Scientific secondary antibodies are conjugated to Horseradish-Peroxidase (HRP) for use in Western blot applications. These antibodies are affinity-purified and optimized for use in ECL experiments and have been absorbed against the serum proteins of a large number of species to virtually eliminate background and non-specific staining.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Requirement of essential Pbp2x and GpsB for septal ring closure in Streptococcus pneumoniae D39.
SA1-100 was used in western blot to study the roles of Pbp2x and GpsB in Streptococcus pneumoniae septal ring closure
|Land AD,Tsui HC,Kocaoglu O,Vella SA,Shaw SL,Keen SK,Sham LT,Carlson EE,Winkler ME||Molecular microbiology (90:939)||2013|