Western blot analysis was performed on whole cell extracts (30 µg lysate) of K562 (Lane 1) and U-87 MG (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product# MA1-106, 2 µg/mL) and detected using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP (Product # 31431) at dilutions 1:5,000 (Fig. 1), 1:50,000 (Fig. 2) and 1:1,00,000 (Fig. 3). A 24 kDa band corresponding to SOD2 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 15mg/ml BSA, 50mM sucrose|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1:5,000 - 1:100,000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:5,000 - 1:100,000|
|Immunoprecipitation (IP)||1:500 - 1:5,000|
|Western Blot (WB)||1:5,000 - 1:1,00,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product # 31431 has been successfully used in Western blot, IHC and IP applications.
Product # 31431 reacts with the heavy chains of mouse igg and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Reconstitute with 0.2 ml of distilled water (1 mg/ml after restoration).
Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics.
31431 was used in western blot to propose using three-dimensional collagen scaffolds to study anti-glioma therapeutics
|Lv D,Yu SC,Ping YF,Wu H,Zhao X,Zhang H,Cui Y,Chen B,Zhang X,Dai J,Bian XW,Yao XH||Oncotarget (7:56904)||2016|
Inhibition of autophagy using 3-methyladenine increases cisplatin-induced apoptosis by increasing endoplasmic reticulum stress in U251 human glioma cells.
31431 was used in western blot to ascertain the role of autophagy in cisplatin chemotherapy
|Zhang R,Wang R,Chen Q,Chang H||Molecular medicine reports (12:1727)||2015|
GSTP1 arrests bladder cancer T24 cells in G0/G1 phase and up-regulates p21 expression.
31431 was used in western blot to assess the effects of GSTP1 overexpression in bladder cancer cells
|Gao L,Fang YQ,Zhang TY,Ge B,Xu B,Huang JF,Zhang ZF,Tan N||International journal of clinical and experimental medicine (7:2984)||2014|
Distribution of vitellogenin in zebrafish (Danio rerio) tissues for biomarker analysis.
31431 was used in western blot to assess the expression of vitellogenin and its receptor in zebrafish
|Zhong L,Yuan L,Rao Y,Li Z,Zhang X,Liao T,Xu Y,Dai H||Aquatic toxicology (Amsterdam, Netherlands) (149:1)||2014|
|Not Applicable||Not Cited||
Use of the disulfiram/copper complex for breast cancer chemoprevention in MMTV-erbB2 transgenic mice.
31431 was used in immunohistochemistry - paraffin section and western blot to test the effect of disulfiram/copper complex on breast cancer cells
|Yang Y,Deng Q,Feng X,Sun J||Molecular medicine reports (12:746)||2015|