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Immunofluorescent analysis of Phalloidin (dark red) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 680 Phalloidin (Product # 21839) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:25 - 1:100|
|Immunocytochemistry (ICC)||1:50 - 1:2,000|
|Immunofluorescence (IF)||1:50 - 1:2,000|
|Immunohistochemistry (IHC)||1:50 - 1:2,000|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:5,000 - 1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Product # 35502 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 35502 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 488 Amax= 493 nm; Emax= 518 nm. Mole Dye/Mole Protein Ratio is lot-dependent.
Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Antiviral activity of baicalin against influenza virus H1N1-pdm09 is due to modulation of NS1-mediated cellular innate immune responses.
35502 was used in immunohistochemistry to study the role of cellular innate immune responses mediated by NS1 in the antiviral mechanism of baicalin against the H1H1 influenza virus from the 2009 pandemic
|Nayak MK,Agrawal AS,Bose S,Naskar S,Bhowmick R,Chakrabarti S,Sarkar S,Chawla-Sarkar M||The Journal of antimicrobial chemotherapy (69:1298)||2014|
Distinctive renal cell tumor simulating atrophic kidney with 2 types of microcalcifications. Report of 3 cases.
35502 was used in immunohistochemistry to report on three patients presenting with renal cell tumor simulating atrophic kidney
|Hes O,de Souza TG,Pivovarcikova K,Grossmann P,Martinek P,Kuroda N,Kacerovska D,Svajdler M,Straka L,Petersson F,Hora M,Michal M||Annals of diagnostic pathology (18:82)||2014|
Brain microvascular accumulation and distribution of the NOTCH3 ectodomain and granular osmiophilic material in CADASIL.
35502 was used in immunohistochemistry to study the microvascular distribution of the Notch3 ectodomain and granular osmiophilic material in the brains of patients with CADASIL
|Yamamoto Y,Craggs LJ,Watanabe A,Booth T,Attems J,Low RW,Oakley AE,Kalaria RN||Journal of neuropathology and experimental neurology (72:416)||2013|
|Not Applicable||Not Cited||
Contribution of increased VEGF receptors to hypoxic changes in fetal ovine carotid artery contractile proteins.
35502 was used in immunohistochemistry to study the role of elevated VEGFR expression in the mechanism of hypoxic vascular remodeling in a fetal ovine carotid artery model
|Adeoye OO,Butler SM,Hubbell MC,Semotiuk A,Williams JM,Pearce WJ||American journal of physiology. Cell physiology (304:C656)||2013|
Method for widespread microRNA-155 inhibition prolongs survival in ALS-model mice.
35502 was used in western blot to study the ability of anti-sense oligonucleotide inhibitors of miRNA-155 to increase survival time in a murine model of ALS
|Koval ED,Shaner C,Zhang P,du Maine X,Fischer K,Tay J,Chau BN,Wu GF,Miller TM||Human molecular genetics (22:4127)||2013|
Selective class IIa histone deacetylase inhibition via a nonchelating zinc-binding group.
35502 was used in western blot to characterize novel trifluoromethyloxadiazole-based inhibitors of class IIa HDACs
|Lobera M,Madauss KP,Pohlhaus DT,Wright QG,Trocha M,Schmidt DR,Baloglu E,Trump RP,Head MS,Hofmann GA,Murray-Thompson M,Schwartz B,Chakravorty S,Wu Z,Mander PK,Kruidenier L,Reid RA,Burkhart W,Turunen BJ,Rong JX,Wagner C,Moyer MB,Wells C,Hong X,Moore JT,Williams JD,Soler D,Ghosh S,Nolan MA||Nature chemical biology (9:319)||2013|