|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.4, with 40% glycerol, 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:50|
|Western Blot (WB)||1:20-1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ZyMAX antibodies are specifically isolated from antigen-affinity columns using advanced elution protocols, leaving only the highest affinity, antigen-specific antibodies. ZyMAX conjugates are prepared with modified cross-linkers to achieve optimal conjugation ratios and stability. Improved purification methods virtually eliminate unconjugated components, giving superior sensitivity and lowest possible levels of background.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phosphorylation of zona occludens-2 by protein kinase C epsilon regulates its nuclear exportation.
62-6511 was used in immunocytochemistry to study localization of zona occludens-2
|Chamorro D,Alarcón L,Ponce A,Tapia R,González-Aguilar H,Robles-Flores M,Mejía-Castillo T,Segovia J,Bandala Y,Juaristi E,González-Mariscal L||Molecular biology of the cell (20:4120)||2009|
The tight junction protein ZO-2 has several functional nuclear export signals.
62-6511 was used in immunocytochemistry to study the nuclear export signals of canine ZO-2
|González-Mariscal L,Ponce A,Alarcón L,Jaramillo BE||Experimental cell research (312:3323)||2006|
The rotavirus surface protein VP8 modulates the gate and fence function of tight junctions in epithelial cells.
62-6511 was used in immunocytochemistry to test if the rotavirus outer capsid proteins are capable of opening the paracellular space sealed by the tight junctions
|Nava P,López S,Arias CF,Islas S,González-Mariscal L||Journal of cell science (117:5509)||2004|
|Not Applicable||Not Cited||
Antigen capture enzyme-linked immunosorbent assay for specific detection of Reston Ebola virus nucleoprotein.
62-6511 was used in immunocytochemistry to develop ELISAs to test for Ebola virus.
|Ikegami T,Niikura M,Saijo M,Miranda ME,Calaor AB,Hernandez M,Acosta LP,Manalo DL,Kurane I,Yoshikawa Y,Morikawa S||Clinical and diagnostic laboratory immunology (10:552)||2003|
||Public can check doctors' background under new US law.||Rutter T||BMJ (Clinical research ed.) (313:null)||1996|
|Not Applicable||Not Cited||Public can check doctors' background under new US law.||Rutter T||BMJ (Clinical research ed.) (313:null)||1996|
|Not Applicable||Not Cited||Delayed formation of the floor plate after ablation of the avian notochord.||Artinger KB,Bronner-Fraser M||Neuron (11:1147)||1993|
|Not Applicable||Not Cited||
Expression of EGF-receptors on epithelial and stromal cells of normal and inflamed gingiva.
62-6511 was used in immunohistochemistry to assess the expression of EGF in normal and inflamed gingival tissues.
|Irwin CR,Schor SL,Ferguson MW||Journal of periodontal research (26:388)||1991|